BACKGROUND Large cohort studies may provide sufficient power to disentangle the role of polymorphisms related to 1-carbon metabolism and chronic diseases, but they require fast, accurate, high-throughput genotyping techniques. MALDI-TOF mass spectrometry has been adapted to rapid fine mapping using various approaches for allele discrimination. We developed a genotyping method based on MALDI-TOF...

The aims of the present study were to propose apolymerase chain reaction-restriction fragment length polymorphismPCR-RFLP genotyping method for the AY_376689:c.773C>T single nucleotide polymorphism SNP of the equine PRKAG3 gene, related to muscle performance, as well as to characterize this SNP and a second polymorphism, AAWR_02017454:g.121684T>C of the SPATA1 gene, related to male fertility, i...

the aims of the present study were to propose apolymerase chain reaction-restriction fragment length polymorphismpcr-rflp genotyping method for the ay_376689:c.773c>t single nucleotide polymorphism snp of the equine prkag3 gene, related to muscle performance, as well as to characterize this snp and a second polymorphism, aawr_02017454:g.121684t>c of the spata1 gene, related to male fertility, i...

the toll-like receptor 4 (tlr4) is an innate immune protein on cell surfaces that identifies lipopolysaccharide (lps) of gram-negative bacteria. genetic markers associated with innate responses during mastitis could help in selection of cattle to improve disease resistance. the objective of this study was to determine the association between tlr4 gene and clinical mastitis, using selective geno...

The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined using reverse transcription-PCR genotyping methods. Common porcine G types G3, G4, and G5 were detected in combination with P types [6] and [7]. The detection of nonporcine G types and unusual G-P combinations and the characterization of an atypical virus indicated that interspecies transmission may co...

Chlamydia trachomatis is one of the most common sexually transmitted infections worldwide. Based on sequence variation in the ompA gene encoding the major outer membrane protein, the genotyping scheme distinguishes 17 recognized genotypes, i.e. A, B, Ba, C, D, Da, E, F, G, H, I, Ia, J, K, L1, L2, and L3. Genotyping is an important tool for epidemiological tracking of C. trachomatis infections, ...

background: during recent years, infection of acinetobacter baumanii showed a rapid growth in hospitals and community. toll-like receptors (tlrs) are the most important pattern recognition receptors, which play a critical role during recognizing invading pathogens by the natural immune system. our objective was to determine the associations of tlrs polymorphisms with the susceptibility to a. ba...

A. 510(k) Number: k093974 B. Purpose for Submission: New Device C. Measurand: Factor II (FII) (Prothrombin) Factor V (FV) Leiden 5, 10 methylenetetrahydrofolate reductase (MTHFR) D. Type of Test: Qualitative genotyping test for single nucleotide polymorphism detection E. Applicant: Osmetech Molecular Diagnostics F. Proprietary and Established Names: eSensor® Thrombophilia Risk Test eSensor® FII...