Most previously reported RNA-cleaving DNAzymes require only a single divalent metal ion for catalysis. We recently reported a general trivalent lanthanide-dependent DNAzyme named Ce13d. This work shows that Ce13d requires both Na(+) and a trivalent lanthanide (e.g. Ce(3+)), simultaneously. This discovery is facilitated by the sequence similarity between Ce13d and a recently reported Na(+)-speci...

Six congenic lines containing B complex recombinants R1 = B-F/B-L24, B-G23; R2 = B-F/B-L2, BG23; R3 = B-F/B-L2, B-G23; R4 = B-F/B-L2, B-G23; R5 = B-F/B-L21, B-G19; and R6R6 = B-F/B-L21, B-G23 were tested individually for antibody response against SRBC. R2, R3 and R4 arose from independent recombination events but are serologically identical. Each B complex recombinant was crossed to inbred Line...

Elderly patients with obstructive lung diseases often receive mechanical ventilation to support their breathing and restore respiratory function. However, mechanical ventilation is known to increase the severity of ventilator-induced lung injury (VILI) in the elderly. Therefore, it is important to investigate the effects of aging to better understand the lung tissue mechanics to estimate the se...

METHODS. Lens epithelial explants and whole lenses from weanling rats were cultured with TGF-/3,, TGF-j32, or TGF-j33 at concentrations ranging from 0.025 ng/ml to 4 ng/ml for 3 to 5 days. Cataractous changes were monitored daily by phase contrast microscopy and by immunofluorescent detection of cataract markers a-smooth muscle actin and type I collagen. Expression of TGF-/3 was studied by immu...

Employment Effects of Payroll Taxes An Empirical Test for Germany* This study tests to what degree the incidence of payroll taxes in Germany is on employment and whether in consequence payroll taxes, in particular social insurance contributions, are the culprit behind the growing unemployment problem. Using industry level data for 18 years (1977-1994) we estimate a system of five interdependent...

While compensation accounts for roughly 90% of K-12 instructional costs, there is little evidence of rational design in these systems. This chapter reviews the nature of teacher compensation systems in developed economies and research on their performance effects. Since these compensation schemes typically arise out of collective negotiations, this chapter also surveys the smaller literature on...

This paper proposes that the decline in federal disability insurance (DI) screening stringency raises the incentive of being uninsured. To support it, we provide a theoretical model and empirical evidence. JEL classification: I13, I18, H31, H51, H55

Most strawberry plants have white flowers and red fruit. We developed a new selection with pink fruit, named it G23. Basic phenotypic data were recorded over years of observation experimentation the flower crown diameter, petal color, rate fruit set, as well skin flesh seed color attachment status, weight shape, soluble solids contents, firmness. found that G23 bloomed stable produced consisten...