نتایج جستجو برای: galactosidases
تعداد نتایج: 262 فیلتر نتایج به سال:
1,6-Dideoxy-6,6-difluoronojirimycin (1) was prepared from an easily accessible and commercially available starting material, methyl 2,3,4-tri-O-benzyl-α-D-glucopyranoside. Key steps of this synthesis are a difluorination reaction using DAST and a reductive amination. The overall yield of the synthesis is 44%. Compound 1 has been tested for its activity as an inhibitor of various glucosidases an...
Activity-based protein profiling has emerged as a powerful tool for visualizing glycosidases in complex biological samples. Several configurational cyclophellitol isomers have been shown to display high selectivity as probes for glycosidases processing substrates featuring the same configuration. Here, a set of deoxygenated cyclophellitols are presented which enable inter-class profiling of β-g...
The thermolabile oc-galactosidase (cy-galactosidase A) and thermostable a-galactosidase (cr-galactosidase B) were separated and purified from human placenta. A homogeneous a-galactosidase B preparation was obtained, but the a-galactosidase A preparation contained small amounts of contaminating protein and various other acid hydrolase activities. Each preparation had a molecular weight of approx...
The techniques of metagenomics have allowed researchers to access the genomic potential of uncultivated microbes, but there remain significant barriers to determination of gene function based on DNA sequence alone. Functional metagenomics, in which DNA is cloned and expressed in surrogate hosts, can overcome these barriers, and make important contributions to the discovery of novel enzymes. In ...
Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectiv...
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