1. Ten rat tissues and organs have been assayed for ,-galactosidase with phenyl ,B-D-galactoside, p-nitrophenyl B-D-galactoside, p-aminophenyl ,B-D-galactoside and 4-methylumbelliferyl fl-D-galactoside as substrates. 2. The relative activities of these tissues are independent ofthe mode ofassay, andmaximum rates ofhydrolysis are not greatly affected by the nature ofthe substrate. 3. Inhibition ...

The transglycosylation behavior and capacity of two clan GH-D α-galactosidases, BoGal36A from the gut bacterium Bacteroides ovatus Aga27A guar plant, was investigated compared. enzymes were screened for ability to use para-nitrophenyl-α-galactoside (pNP-Gal), raffinose locust bean gum (LBG) galactomannan as glycosyl donors with acceptors methanol, propanol, allyl alcohol, propargyl alcohol glyc...

A human galactoside-binding protein with an Mr of 31,000 was cloned from the human HT-1080 fibrosarcoma complementary DNA library. A partial complementary DNA clone containing the complete coding region was characterized and the deduced sequence encodes a polypeptide of 242 amino acids with the characteristics of a carbohydrate-binding protein. The gene coding for the human galactoside-binding ...

The symbiotic, nitrogen-fixing bacterium Sinorhizobium meliloti favors succinate and related dicarboxylic acids as carbon sources. As a preferred carbon source, succinate can exert catabolite repression upon genes needed for the utilization of many secondary carbon sources, including the alpha-galactosides raffinose and stachyose. We isolated lacR mutants in a genetic screen designed to find S....

The levels of flavonoids in blueberries (Vaccinium corymbosum L.) were found to increase after illumination with UV-C. Phytochemicals affected included resveratrol, myricetin-3-arabinoside, quercetin-3-galactoside, quercetin-3-glucoside, kaempferol-3-glucuronide, delphinidin-3-galactoside, cyanidin-3-galactoside, delphinidin-3-arabinoside, petunidin-3-galactoside, petunidin-3-glucoside, petunid...

Exposure of Atlantic salmon smolts to estrogenic compounds is shown to compromise several aspects of smolt development. We sought to determine the underlying endocrine mechanisms of estrogen impacts on the growth hormone (GH)/insulin-like growth factor I (IGF-I) axis. Smolts in freshwater (FW) were either injected 3 times over 10 days with 2 μgg(-1) 17β-estradiol (E2) or 150μgg(-1) 4-nonylpheno...

Three fluorogenic methylumbelliferone (MU) substrates were evaluated for rapid detection of total and fecal coliform bacteria (TC and FC) in drinking water. 4-MU-beta-D-galactoside, MU-heptanoate, and MU-glucuronide were used to determine enzyme activity as a surrogate measure of coliform concentration. Coliforms occurring in river water and in potable water artificially contaminated with raw s...

BACKGROUND Qualitative and quantitative analyses of 'Zonouz' and 'Gala' apples phenolic compounds were accomplished by HPLC. MATERIAL AND METHODS Samples for phenolics study were taken at three different growing stages [1 - fruit early growing stage 2 - mid-stage of fruit development (80 days after full bloom) 3 - during commercial harvest time]. RESULTS The results showed qualitative diffe...