A total of 137 strains of Gardnerella vaginalis were examined by the API 20 Strep system. The system was shown to be reliable when the tests were compared with standard identification methods, and very little confusion occurred with streptococcal profiles; consequently, G. vaginalis has been included in the API 20 Strep data base.

Amounts of putrescine, cadaverine, and tyramine were measured in vaginal washings from five patients with non-specific vaginitis (NSV) associated with Gardnerella vaginalis, five patients with trichomoniasis, and five healthy controls. Putrescine and cadaverine were present in all but one sample from the infected patients; tyramine was found in four of five samples from the women with NSV and i...

The ESwab collection device was compared to the collection swab provided as part of the Affirm VPIII microbial identification test kit for testing vaginal specimens with the Affirm test system. There was excellent agreement between the two sampling devices for Candida spp., Gardnerella vaginalis, and Trichomonas vaginalis.

Presumptive identification of Gardnerella vaginalis from 48-h human blood agar cultures by using a Gram stain, hemolysis, and colonial morphology was highly accurate.

CONTEXT Vaginitis is one of the principal motives that lead women to seek out an obstetrician or gynecologist. Bacterial vaginosis, candidiasis and trichomoniasis are responsible for 90% of the cases of infectious vaginitis. OBJECTIVE To verify the frequency of the three main causative agents of vaginitis, Trichomonas vaginalis, Candida sp and Gardnerella vaginalis, in four different decades ...

Vaginal discharge is a common complaint of women attending gynaecological clinics. The purpose of this study was to compare the occurrence of commonly implicated microorganisms in vaginal discharge amongst women with or without the complaint, attending a gynaecological and family planning clinic. The association of Gardnerella vaginalis with bacterial vaginosis was also studied. It was found th...

Western blot analysis was used to identify antigenic components of Gardnerella vaginalis. Polypeptides bound to nitrocellulose membranes were probed with murine antisera raised to two strains of G. vaginalis, and antibody-antigen complexes were detected with 125I-labelled antimouse immunoglobulin followed by autoradiography. Although there was inter-strain variation in immunogenic polypeptide p...