BACKGROUND Recessive mutations in guanylate cyclase-1 (Gucy2d) are associated with severe, early onset Leber congenital amaurosis-1(LCA1). Gucy2d encodes guanylate cyclase (GC1) is expressed in photoreceptor outer segment membranes and produces cGMP in these cells. LCA1 patients present in infancy with severely impaired vision and extinguished electroretinogram (ERG) but retain some photorecept...

Background NO/cGMP-mediated signal transduction is involved in synaptic plasticity in various brain regions. NO effects are transduced by the NO receptor guanylyl cyclase (NO-GC) that exists in two isoforms, NO-GC1 and NO-GC2, with indistinguishable regulatory properties. Mice deficient in either NO-GC1 or NO-GC2 revealed that both NO-GC isoforms are required for LTP indicating the existence of...

A biochemical analysis of glycoprotein C (gC of herpes simplex virus was undertaken to further characterize the structure of the glycoprotein and to determine its disulfide bond arrangement. We used three recombinant forms of gC, gC1(457t), gC1(delta33-123t), and gC2(426t), each truncated prior to the transmembrane region. The proteins were expressed and secreted by using a baculovirus expressi...

The Gluconobacter phage GC1 is a novel member of the Tectiviridae family isolated from a juice sample collected during dry white wine making. The bacteriophage infects Gluconobacter cerinus, an acetic acid bacterium which represents a spoilage microorganism during wine making, mainly because it is able to produce ethyl alcohol and transform it into acetic acid. Transmission electron microscopy ...

OBJECTIVES To explore the cause of third-generation cephalosporin resistance in Australian Acinetobacter baumannii isolates belonging to global clone 1 (GC1). METHODS GC1 isolates from Australia were tested for resistance to ceftazidime and cefotaxime using disc diffusion and MICs. PCR was used to determine the context of ISAba1-ampC configurations and amplicons were sequenced. The level of t...

OBJECTIVES To locate the acquired bla(OXA-23) carbapenem resistance gene in an Australian A. baumannii global clone 1 (GC1) isolate. METHODS The genome of the extensively antibiotic-resistant GC1 isolate A85 harbouring bla(OXA-23) in Tn2006 was sequenced using Illumina HiSeq, and the reads were used to generate a de novo assembly. PCR was used to assemble relevant contigs. Sequences were comp...

Photoreceptor (PR) cells are highly specialized cells that convert light into electrical signals. Ten percent of their outer segment (OS) membranes (approximately 77 cm2 of membrane) are renewed every day. Therefore, PR cells must possess an extraordinary trafficking system to provide all of the needed material to build up the OS discs through a 0.3 µm diameter connecting cilium. The mechanism ...

Background Alterations in vascular relaxation have been reported in various models of hypertension. With its tonic activity, the NO/cGMP signalling cascade plays an important role in the regulation of the vascular tone. In blood vessels, NO is continuously being produced by the endothelial NO synthases (eNOS) which leads to cGMP production by stimulating the NO-sensitive guanylyl cyclases (NO-G...

Photoreceptor ROS-GC1, a prototype subfamily member of the membrane guanylate cyclase family, is a central component of phototransduction. It is a single transmembrane-spanning protein, composed of modular blocks. In rods, guanylate cyclase activating proteins (GCAPs) 1 and 2 bind to its juxtamembrane domain (JMD) and the C-terminal extension, respectively, to accelerate cyclic GMP synthesis wh...

Abstract. The Arctic's glacial history has classically been interpreted from marine records in terms of the fluctuations Eurasian and North American ice sheets. However, extent timing East Siberian Ice Sheet (ESIS) have remained uncertain. A recently discovered glacially scoured cross-shelf trough extending to edge continental shelf north De Long Islands provided additional evidence that existe...