OBJECTIVES To determine the genome sequence of Acinetobacter baumannii strain MDR-TJ and characterize the mechanisms of multidrug resistance in this strain. METHODS The whole-genome sequence was determined using Roche 454 GS FLX Titanium. Subsequently, the gaps were closed by sequencing PCR products. The genome of strain MDR-TJ was annotated using IMG ER, the RAST annotation server and the BA...

Lipooligosaccharide (LOS) is a complex surface structure that is linked to many pathogenic properties of Acinetobacter baumannii. In A. baumannii, the genes responsible for the synthesis of the outer core (OC) component of the LOS are located between ilvE and aspS. The content of the OC locus is usually variable within a species, and examination of 6 complete and 227 draft A. baumannii genome s...

Objectives To investigate the context of the ribosomal RNA methyltransferase gene armA in carbapenem-resistant global clone 2 (GC2) Acinetobacter baumannii isolates from Singapore. Methods Antibiotic resistance was determined using disc diffusion; PCR was used to identify resistance genes. Whole genome sequences were determined and contigs were assembled and ordered using PCR. Resistance regi...

Multiple sclerosis (MS) is one of the most common causes of neurological disability in early adulthood. The current literature is interested in identifying biological or DNA markers associated with genetic susceptibility to MS. The aim of this study is to investigate, by means of Bayesian statistical inference, whether the presence of Gc2 (Gc = group-specific component) and/or EsD1 (EsD = ester...

for (Z',Xk') $ (Zk , Xk"), A , Zk < 1 (lb). For every point (Z1,Z20), ZK0 = hKk(ZkoXko) ZkAol < 1, and for every sufficiently small neighborhood U of this point, there exists o> 0 such that the set of points of b3 which belong to U consists only of points (1) for which Zk-Zkol < a and XXkol < a hold, (lc). For a fixed k and Xk, the corresponding set of points (1) is called a lamina of ek3 and i...

Sir, Resistance to third-generation cephalosporins such as ceftazidime and cefotaxime in Acinetobacter baumannii is often due to increased expression of an intrinsic ampC gene. Expression increases when the ISAba1 insertion sequence (IS) is present in the appropriate orientation upstream of the ampC gene, providing an outward-facing promoter that appears to be stronger than the intrinsic promot...

BACKGROUND We investigated the whole genome sequence (WGS) of a carbapenem-resistant Acinetobacter baumannii isolate belonging to the global clone 2 (GC2) and predicted resistance islands using a software tool. METHODS A. baumannii strain YU-R612 was isolated from the sputum of a 61-yr-old man with sepsis. The WGS of the YU-R612 strain was obtained by using the PacBio RS II Sequencing System ...

A genital herpes vaccine is urgently needed to prevent pain and suffering, reduce the incidence of neonatal herpes, and decrease the risk of HIV acquisition and transmission that accompanies genital infection. We evaluated a trivalent HSV-2 subunit antigen vaccine administered with CpG and alum in rhesus macaques and guinea pigs. The vaccine contains glycoproteins C, D and E (gC2, gD2, gE2) to ...

A set T ⊂ R at which interpolation from Π≤n(R ) (polynomials of degree ≤ n) is uniquely possible is a GCn-set if the associated Lagrange fundamental polynomials have only linear factors. For such GCn-sets T in the plane, Gasca and Maeztu conjectured the existence of a line containing n + 1 points from T. It is shown here that, already in R, there exist GC2-sets T without any hyperplanes contain...