Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The ...

Using Agrobacterium rhizogenes due to create hairy roots is a useful method to product secondary metabolites in many medicinal plants. The transgenic hairy roots were induced from Peganum multisectum (Maxim) Bobrov a medicinally important species, by infecting leaf and stem explant with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 19% of ...

Shoot regeneration in safflower (Carthamus tinctorius 'AKS 207' and 'PKV Pink') genetically transformed using Agrobacterium was used for assessing various constraints to the efficiency of transformation including infection period, virulence induction medium, co-cultivation period, bacterial titre, selection regime, and the natural phenolic compound acetosyringone. Transformation frequency was p...

It is an increased interest in the therapeutic potential of Cannabis sativa L. (marijuana) for treatment of multiple sclerosis and HIV neuropathy. Because of limitation in cultivation of this plant, an efficient hairy root induction system for Cannabis sativa L. was developed in the present study. Agrobacterium rhizogenes mediated transformation performed by two different co-cultivation mediums...

RNAi constructs targeted to four different genes were examined to determine their efficacy to reduce galls formed by Meloidogyne incognita in soybean roots. These genes have high similarity with essential soybean cyst nematode (Heterodera glycines) and Caenorhabditis elegans genes. Transformed roots were challenged with M. incognita. Two constructs, targeted to genes encoding tyrosine phosphata...

Using Coreopsis tinctoria liquid cultures of Agrobacterium rhizogenes transformed roots (socalled “hairy roots”) and non-transformed roots have been established. The production of differ­ ent phenylpropanoid compounds by these cultures grown in light or darkness, respectively, was compared. Whereas the composition of phenylpropanoids in the root cultures was not influenced by the culture condit...