We showed that brief exposures to amphotericin B (AmB) inhibited the induction of new Candida germ tubes and the lengthening of partially induced germ tubes. Blastoconidia with germ tubes were more susceptible to AmB killing, and this varied directly with the induction period and the AmB exposure period. AmB did not preferentially affect germ tube adherence to fibrin matrices.

We report on the elucidation of two separate pathways of spore germination in a plant pathogenic fungus Colletotrichum gloeosporioides f. sp. aeschynomene. Conidia of the fungus can germinate either from one side or from both sides, depending on external conditions. In shake culture that includes an extract made up from fresh peas, the unicellular conidium divides and one of the two cells devel...

Candida albicans is a pathogenic dimorphic fungus. When yeast cells were pre-incubated in YPD medium at 25degreesC and released into HFM7 medium containing 4% serum at 37degreesC, germ tubes emerged within 0.5 h. To determine whether mRNA or protein synthesis was necessary for germ tube formation, we examined the effects of mRNA and protein syntheses inhibitors on this formation. In the presenc...

This study was performed to investigate the use of chromogenic tube and methyl blue-Sabouraud agar for the presumptive identification of Candida albicans. 124 clinical isolates, including 111 C.albicans and 13 Candida spp strains, which had been identified by morphology on cornmeal tween 80 agar and Vitek automated identification system, were included. Three different identification procedures,...

JRK’s DOCD is a proprietary siddha medicine, indicated for prevention of co-morbidities diabetes. Earlier studies on the product has proven its effect in boosting phagocyte mediated immunity. In current study ability preventing pathogenicity fungi evaluated. Germ tube formation to increase than spores alone. For this different species i.e., Candida albicans, 2 isolates dubelensis and three Muco...

Indirect immunofluorescent and agglutination assay were used to study the anti-Candida albicans reactivities in the serum of 13 normal subjects and 14 patients infected with C. albicans. A significant increase in anti-C. albicans seroreactivity was observed during infection with this organism but the increase in the anti-germ tube immunofluorescence titre was the more marked. It is evident that...

475 Abstract: The pattern of distribution of fibronectin adhesins on yeast and hyphalform Candida albicans was studied with goldlabelled fibronectin and transmission electron microscopy. The germ-tube parts of the hyphal-form cells were the major binding sites for gold-labelled fibronectin, and the attached yeast-cell parts and yeast-form cell remained unlabelled. Therefore, the walls of the ge...

We implemented a simple, sensitive, objective, and rapid cellular assay to reveal the antifungal activity of a novel class of glucan synthase inhibitors. The assay, especially useful for early drug discovery, measures the transformation of Candida albicans from the yeast form to the hyphal form. Test compounds were ranked by potency (50% inhibitory concentration) and efficacy (percent inhibitio...

BACKGROUND Candida albicans is the most pathogenic Candida species but shares many phenotypic features with Candida dubliniensis and may, therefore, be misidentified in clinical microbiology laboratories. Candidemia cases due to C. dubliniensis are increasingly being reported in recent years. Accurate identification is warranted since mortality rates are highest for C. albicans infections, howe...

The protein kinase A (PKA) signaling pathway plays a role in regulating growth and differentiation in the dimorphic fungus Mucor circinelloides. PKA holoenzyme is comprised of two catalytic (C) and two regulatory (R) subunits. In M. circinelloides, four genes encode the PKAR1, PKAR2, PKAR3, and PKAR4 isoforms of R subunits. We have constructed null mutants and demonstrate that each isoform has ...