the chromosomal spread and karyotype of bleak (alburnus alburnus) from anzali lagoon were identified using tissue squashing techniques with injection of 0.5ml/100g body weight of 0.01% colchicines solution in fish fingerlings. kidney and gill tissues were then extracted and chopped in kcl 0.045m for 20 min and fixed in carnoy solution in 3 stages. the chromosomal spreads were stained in 20% gim...

OBJECTIVE To analyze the amount and inflammatory components of the sputum in patients with "disharmony of stomach", including gastroesophageal reflux disease, peptic ulcer and chronic gastritis. METHODS Sputum production was induced by inspiration of aerosol of 3.5% hypertonic saline with ultrasonic nebulizer, and the total cell count in the sputum was examined. Sputum smear stained with Gims...

Background: Fluorescence Imitating Brightfield Imaging (FIBI) is a novel alternative microscopy method that can image freshly excised, non-sectioned tissue.We examine its potential utility in dermatopathology. Methods: Five skin samples embedded paraffin blocks were superficially deparaffinized using xylene and ethanol then briefly stained with hematoxylin eosin (H&E). FIBI captured tissue surf...

BACKGROUND/AIM To date, the conventional formalin-fixed, paraffin-embedded (FFPE) technique is the gold-standard for preserving histomorphology. Once FFPE tissues are stained, slides are routinely archived along with their blocks at biobanks/hospitals. However, the reuse of fixed and stained biospecimens as DNA source is not a common routine practice worldwide and, thus, indicates the need of s...

It is presented the case of an old female with a solitary tumor at the level of the thorax. The specimen was processed using the routine histological technique and slides were stained with conventional morphologic, histochemical and immunohistochemical methods. On Hematoxylin-Eosin stained slides were noticed large cells with acidophilic cytoplasm, with granular pattern. S100 protein was intens...

A new staining method for dual demonstration of Estrogen receptors (ER) and argyrophilc Nucleolus-Organizer Regions (AgNORs) was developed. To rule out possible reciprocal effects, serial slides of 10 invasive ductale breast cancers were stained with either the single staining method or the simultaneous ER/AgNOR-staining method and investigated comparatively. By measuring the slides with the im...

Examination of chromogenically stained tissues has been the hallmark of clinical diagnostic pathology and cancer research for nearly a century. Fixed and stained tissue on microscope slides has been the routinely used method of data storage. Pathological samples to be viewed were routinely fixed and embedded in paraffin for long term storage. Thin slices were then made from the paraffin samples...