A new glucanolytic bacterial strain, SU40 was isolated, and identified as Bacillus subtilis on the basis of 16S rRNA sequence homology and phylogenetic tree analysis. The gene encoding β-1,3-1,4-glucanase was delineated, cloned into pET 28a+ vector and heterologously overexpressed in Escherichia coli BL21(DE3). The purified recombinant enzyme was about 24 kDa. The enzyme exhibited maximum activ...

1. A cellobiohydrolase component was isolated from a Penicillium funiculosum cellulase preparation by chromatography on DEAE-Sephadex, and purified by isoelectric focusing. 2. Purified in this way, the enzyme was homogeneous as judged by electrophoresis on sodium dodecyl sulphate/polyacrylamide gels and isoelectric focusing in polyacrylamide gels. 3. Acting in isolation, the enzyme had little h...

A gene encoding a beta-1,3-1,4-glucanase (CelA) belonging to family 5 of glycoside hydrolases was cloned and sequenced from the Bacillus subtilis A8-8. The open-reading-frame of celA comprised 1499 base pairs and the enzyme was composed of 500 amino acids with a molecular mass of 55 kDa. The recombinant beta-1,3-1,4 glucanase was purified by GST-fusion purification system. The pH and temperatur...

Gliocladium virens is a common saprophytic fungus that is mycoparasitic on a large number of fungi. Responses of G. virens toward its environment were examined by monitoring the presence of extracellular proteins in culture fluid during time course experiments. Culture fluid of G. virens grown on glucose, washed cell walls of Rhizoctonia solani (one of its hosts), olive oil, or chitin contained...

Cellulosimicrobium cellulans (also known with the synonyms Cellulomonas cellulans, Oerskovia xanthineolytica, and Arthrobacter luteus) is an actinomycete that excretes yeast cell wall lytic enzyme complexes containing endo-beta-1,3-glucanases [EC 3.2.1.39 and 3.2.1.6] as key constituents. Three genes encoding endo-beta-1,3-glucanases from two C. cellulans strains have been cloned and characteri...

"e studies have been performed to analyze the production of β-glucanase by a recombinant strain of Escherichia coli immobilized in di#erent matrices. Porous sintered glass SIRAN®, Ceramic supporting matrices and Broken Pumice stone as well as SIRAN Raschig-rings were examined for the immobilization of whole bacterial cells. "e β-glucanase activity of bacteria immobilized in CeramTec PST 5 (4–5 ...

brassica napus is an important oilseed crop and the yield loss due to fungal disease stem rot caused by sclerotinia sclerotiorum is a serious problem in cultivation of this crop. the pathogenesis-related (pr) protein, glucanase, hydrolyzes a major cell wall component, glucan, of the pathogenic fungi and acts as a plant defense barrier. in this study, a β-1,3-glucanase (bgn13.1) gene was isolate...

Selected hydrolytic enzymes are added to animal feeds in order to degrade specific antinutritional factors and(or) to increase availability of certain components of feedstuffs to the animal. A method is described that allows detection and quantification of beta-glucanase activity in complex feedstuffs. The method is based on radial diffusion of an enzyme-containing feed extract through an agar ...

a total number of  5520 female and  480 male breeders (arbor acres plus) at 42wks of age  were used in a 10-week-trial to investigate the effect of an enzyme complex on  the quality  and quantity of egg production as well as hatchability of broiler breeders fed a corn-soybean meal diet. there were two dietary treatment groups: a control group fed on a corn-soybean based diet, and the multi-enzy...