A strain of bakers' yeast was isolated which could utilize cellobiose and other beta-D-glucosides quantitatively as carbon and energy sources for growth. Cellobiose-grown cells contained a largely cryptic enzyme active against the chromogenic substrate p-nitrophenyl-beta-D-glucoside. The patent (intact cell) activity of such cells was inhibited by azide and, competitively, by cellobiose; neithe...

background: the mechanism of hypoglycemic and hypolipidemic activities of pyrus biossieriana buhse leaf extract (pbble) and its phytochemical component arbutin, have not been well determined. the present study was performed to understand the hypoglycemic activity mechanisms of pbble and arbutin more clearly.methods: in vitro enzymatic carbohydrate digestion with pbble and arbutin was assessed u...

it is believed that the inhibition of carbohydrate hydrolyzing enzymes including α-amylase and α-glucosidase is one of the therapeutic approaches to decrease the postprandial glucose level after a meal, especially in the people with type 2 diabetes. medicinal plants and their extracts are one of the main sources to find new inhibitors to the enzymes. in our study four flavonoids, namely luteoli...

the colorado potato beetle, leptinotarsa decemlineata say (col.: chrysomelidae) is an important pest of potato worldwide and study of its glucosidases is an important step to develop appropriate pest control strategies. in this study, some biochemical aspects of β-glucosidase in the colorado potato beetle were determined. the results showed that β-glucosidase activity in the midgut of adults wa...

Most biological fluids contain both neutral and acid alpha-glucosidase. Optimal conditions were therefore developed for the selective determination of the activity of neutral and acid alpha-glucosidase, using 2-step, discontinuous assays. In the first step of the assay of neutral alpha-glucosidase, glucose was liberated from maltose (citrate-phosphate buffer, pH 6.8, 20 mmol/l maltose, 25 mmol/...

Lactobacillus delbrueckii mutant Uc-3 utilizes both cellobiose and cellotriose efficiently, converting it into L(+) lactic acid. The enzyme activities of cellobiose and cellotriose utilization were determined for cell extracts, whole cells, and disrupted cells. Aryl-beta-glucosidase activity was detected only for whole cells and disrupted cells, suggesting that these activities are cell bound. ...

Background Compost habitats sustain a vast ensemble of microbes that engender the degradation of cellulose, which is an important part of global carbon cycle. β-Glucosidase is the rate-limiting enzyme of degradation of cellulose. Thus, analysis of regulation of β-glucosidase gene expression in composting is beneficial to a better understanding of cellulose degradation mechanism. Genetic diversi...

In the current study, we screened 7 clonal lines from single seed phenotypes of Lamiaceae family for the inhibition of alpha-amylase, alpha-glucosidase and angiotensin converting enzyme (ACE) inhibitory activity. Water extracts of oregano had the highest alpha-glucosidase inhibition activity (93.7%), followed by chocolate mint (85.9%) and lemon balm (83.9%). Sage (78.4 %), and three different c...

The aand l-glucosidase activity in natural samples can be readily measured during short incubation times (20 min) by using the artificial substrates 4-methylumbelliferyl-a-D-glucoside and 4-methylumbelliferyli--Dglucoside. The apparent Km of both aand ,I-glucosidase for these respective substrates is 0.01 ,uM. The homologous disaccharides maltose and cellobiose competitively inhibit aand I-gluc...