D. Stuber and K. Henco. 1994. 6xHis-NiNTA chromatography as a superior technique in recombinant protein expression/purification. Methods Mol. Biol. 31:371-387. 3.Galibert, F., E. Mandart, F. Fitoussi, P. Tiollais and P. Charnay. 1979. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature 281:646-650. 4.Gentz, R., C.-H. Chen and C.A. Rosen. 1989. Bioassay fo...

---There has been escalation in alcohol abuse with an enormous increase in alcohol-related gastrointestinal tract disorders. The study was conducted to evaluate the role of zinc on intestine of rats under ethanol toxicity. Rats were segregated into four groups: Control, Ethanol treated, Zinc treated and Combined zinc and ethanol treated. 1ml of 30% ethanol was given to rats orally everyday. Zin...

Copyright  2015 irjpbs. Aim of the present study is to evaluate the preventive role of phytic acid on mitochondrial lipid peroxides, antioxidants and lipids in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats subcutaneously injected with ISO (85 mg/kg) at an interval of 24 h for 2 days resulted in a significant increase in the levels of mitochondrial lipid perox...

Background: Alteration in oxidative and nitrosative stress as well as antioxidant status is known to occur in carcinogenesis and during treatment of cancer with chemotherapeutic drugs. Objective: The objective of this study was to evaluate the serum levels of reduced glutathione (GSH) glutathione-stransferase (GST) and nitric oxide (NO • ) in post-operative stage II breast cancer patients under...

Allitinib, a novel irreversible selective inhibitor of the epidermal growth factor receptor (EGFR) 1 and human epidermal receptor 2 (ErbB2), is currently in clinical trials in China for the treatment of solid tumors. It is a structural analog of lapatinib but has an acrylamide side chain. Sixteen metabolites of allitinib were detected by ultra-high-performance liquid chromatography/quadrupole t...

Background: Hemidesmus indicus is an important medicinal plant and extensively used in Ayurvedic and Unani system of medicine. The aim of the study was to evaluate the antioxidant, hypoglycaemic and hypolipidemic potential of methanolic extract of roots of Hemidesmus indicus (HIE) in streptozotocin (STZ) induced diabetic mice. Methods: HIE was analyzed by LC-MS to determine its phytochemical co...

Propionic acid (PA) is a short chain fatty acid, a common food preservative and metabolic end product of enteric bacteria in the gut. The present study was undertaken to investigate the effect of PA on liver injury in male rats. Male western albino rats were divided into two groups. The first group served as normal control, the second was treated with PA. The activities of serum hepatospecific ...

Transcriptional activation of the soybean (Glycine max) C H Z / 4 gene (also referred to as GmhspZ6-A) and increase in abundance of the C H Z / 4 mRNA (also referred to as pCE54) have been previously shown to occur following treatment of soybean seedlings with auxins, nonauxin analogs, heavy metals, and a variety of other agents. To determine whether the G H Z / 4 promoter is responsive to an a...

We previously reported that a velvetleaf (Abutilon theophrasti Medic) biotype found in Maryland was resistant to atrazine because of an enhanced capacity to detoxify the herbicide via glutathione conjugation (JW Gronwald, Andersen RN, Yee C [1989J Pestk Blochem Physiol 34: 149-163). The biochemical basis for the enhanced atrazine conjugation capacity in this biotype was examined. Glutathione le...