Membranous microparticles (MP) appearing in the supernatant plasma of stored platelet concentrates (PC) were analyzed by flow cytometry. Two populations of MP were arbitrarily delineated by light scatter as larger or smaller than 0.5 micron fluorescent beads. An estimate of MP concentration was obtained by adding a known amount of fluorescent beads to each sample before analysis of a set number...

Gastric infection by Helicobacter pylori is considered a risk factor for gastric and duodenal cancer, and extragastric diseases. Previous data have shown that, in a non-enzymatic way, H. pylori urease (HPU) activates neutrophils to produce ROS and also induces platelet aggregation, requiring ADP secretion modulated by the 12-lipoxygenase pathway, a signaling cascade also triggered by the physio...

Abstract Background A strong relationship links migraine with aura (MHA) and patent foramen ovale (PFO). Increased platelet aggregation oxidative stress were documented in migraineurs. To date, no mechanisms connecting MHA to PFO have been demonstrated. Objectives perform a comprehensive analysis of activation, inflammation, status 78 aspirin-treated MHA-patients before (T0) 6-months after (T1)...

BACKGROUND Medical decision making must often be performed despite incomplete evidence. An example is the choice of a glycoprotein IIb/IIIa (GP2b3a) inhibitor, a class of potent antiplatelet medications, as adjunctive therapy during percutaneous coronary interventions (PCIs). GP2b3a inhibitor efficacy in reducing adverse outcomes has been well documented with multiple placebo-controlled randomi...

Thrombin potently regulates human platelets by the G protein-coupled receptors protease-activated receptor (PAR) 1 and PAR4. Platelet activation by thrombin and other agonists is broadly inhibited by prostacyclin and nitric oxide acting through adenylyl and guanylyl cyclases to elevate cAMP and cGMP levels, respectively. Using forskolin and YC-1 [3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole] ...

objective(s): chemoresistance remains the main causes of treatment failure and mortality in cancer patients. there is an urgent need to investigate novel approaches to improve current therapeutic modalities and increase cancer patients' survival. induction of drug efflux due to overexpression of                  p-glycoproteins is considered as an important leading cause of multidrug resis...

Background: Magnetic Resonance Imaging (MRI) is a powerful non-invasive technique that provides highresolution anatomical images of biological tissue based on their inherent physio-chemical properties. The signal from MRI can be modulated by contrast agents, which can be specifically directed to cells and molecules of interest, thereby providing an opportunity for targeted imaging. Aims: We pro...

Essentials Platelets play an important role in pathogen recognition. Platelets contain several complement factors and can interact with E. coli. Platelet's complement protein C3 differs from plasmatic C3 in its electrophoretic mobility. Upon contact with bacteria, platelets are activated and can enhance complement activation. SUMMARY Background The role of platelets in immune defense is incre...