OBJECTIVE The aim of the study was to isolate gossypol-degrading bacteria and to assess its potential for gossypol degradation. METHODS Rumen liquid was collected from fistulated cows grazing the experimental pasture. Approximately 1 mL of the rumen liquid was spread onto basal medium plates containing 2 g/L gossypol as the only source of carbon and was then cultured at 39°C to isolate gossyp...

Gossypol is a polyphenolic compound originally isolated from cottonseed that has long been used successfully as a contraceptive drug for males. However, the molecular mechanism of anti-telomerase effects of gossypol is not entirely understood. In this study, we observed direct cytotoxicity and inhibition of telomerase activity with downregulation of telomerase reverse transcriptase (hTERT) expr...

Plasma and tissue concentrations of total, (+)- and (-)-gossypol were determined in broilers fed cottonseed meals (CSM) from eight oil mills (five expander solvent, two expeller, and one direct solvent). Free gossypol in the meals ranged from 0.033 to 0.180%, and total gossypol ranged from 0.974 to 1.459%. The (+)-enantiomer of gossypol varied from 53.8 to 61.3% of total gossypol. Eight CSM die...

Aberrant overexpression of antiapoptotic members of the Bcl-2 protein family, including Bcl-2 and Bcl-X(L), contributes to malignant transformation and subsequent resistance to traditional chemotherapeutics. Thus, these proteins represent attractive targets for novel anticancer agents. The small molecule, gossypol, was initially investigated as a contraceptive agent, but subsequently has been s...

( )-Gossypol, a natural BH3-mimetic and small-molecule Bcl-2 inhibitor, shows promise in ongoing phase II clinical trials for human cancers. However, whether ( )-gossypol plays functional roles in tumor angiogenesis has not been directly elucidated yet. In this study, we showed that ( )-gossypol dose dependently inhibited the expression of VEGF, Bcl-2, and Bcl-xL in human prostate cancer cells ...

Gossypol is a yellow pigment occurring in all parts of cotton plants, with the highest levels found in seeds, and it exhibits a variety of toxic effects. Few data are available on the content of gossypol in the commercial complementary feed and in feed raw materials. The present study was focused on the investigation of the presence of free gossypol in commercial complementary feed not containi...

Cottonseed is an economical source of protein and is commonly used in balancing livestock rations; however, its use is typically limited by protein level, fat content, gossypol, and the potential for aflatoxin contamination. There are numerous studies in the literature discussing gossypol and aflatoxin toxicities in livestock and processing methods for reducing gossypol levels in cottonseed. Ho...

Intrinsic fluorescence of LDH-C4 has been studied in the presence of optical isomers of gossypol. The study showed that fluorescence due to tryptophan residues after excitation of LDH at 282 nm is quenched by each gossypol enantiomere in a concentration dependent manner. Half of the maximum quench (Q50%) of enzyme occurred with gossypol (-) at 0.9 x 10(-4) mol/l and with gossypol (+) at 1.4 x 1...

The present paper deals with study of In-vitro cytotoxicity effect of isolated gossypol from Bt and Non-Bt cotton seeds on HeLa cell lines. Gossypol is a polyphenolic binaphthyl diadehyde natural yellow colored pigment. It is not only resistance substance for cotton plant’s self – defense system against insect pests and possibly some diseases but also an important phytochemical compound of imme...

Based on the previous observations that gossypol is biosynthetically formed from acetate, and from the location of the incorporated 1% atoms, gossypol biosynthesis via the isoprenoid pathway has been postulated. To support this hypothesis, the incorporation of mevalonate-2J4C (a key intermediate in the isoprenoid pathway) into gossypol as well as the distribution of the radioactivity in the gos...