background: the transcription factor oct-4, is an important marker of undifferentiating level and a key regulating factor for maintenance of pluripotency in cells. establishment of an oct-4 promoter-based reporter system is an appropriate tool for monitoring the differentiation of embryonic stem cells both in vivo and in vitro. methods: in the present study, we report construction of a recombin...

abstract background: determination of transgenic embryos from non transgenic embryos sibling is an important step in producing homozygous transgenic mice. these steps need by pcr or southern blotting followed extraction of dna, but both techniques require skill and consume time. objective: the aim of this study was simulation of high accuracy method using novel enhanced green fluorescent protei...

Intercellular adhesion molecule-4 (ICAM-4), a newly characterized adhesion molecule, is expressed early in human erythropoiesis and functions as a ligand for binding alpha4beta1 and alphaV integrin-expressing cells. Within the bone marrow, erythroblasts surround central macrophages forming erythroblastic islands. Evidence suggests that these islands are highly specialized subcompartments where ...

in order to evaluation the effects of different soil fertility systems on chickpea seed quality, field experiments were carried out in sanandaj agriculture research station of iran in 2007 and 2008 growing seasons. experimental units were arranged in split-split plots based on randomized complete blocks with three replications. green manure levels considered main plots, base fertilizer compound...

purification and isolation of cellular target proteins for monoclonal antibody (mab) production is a difficult and time-consuming process. immunization of mice with murine cell lines stably transfected with genes coding for xenogenic target molecules is an alternative method for mouse immunization and mab production. here we present data on transfection efficiency of some commercial reagents us...

one of the most effective ways to reach yield stability with low input is increasing diversity through utilizing intercrops in agro-ecosystems. a field experiment based on a randomized complete block design with six treatments and three replications and two crops, green pea and nutrifed millet, was carried out at research farm of agriculture and natural resources of kerman during growing season...