The most common hypochrom microcytic anemia are iron deficiency anemia (IDA) and thalassemia minor (TM). The results of some studies have shown that IDA can cause misdiagnosis of heterozygote β-thalassemia due to decrease in HbA2 level. Our aim in this study was evaluating the effect of IDA on HbA2 levels; Furthermore hematolagic values in CBC of these two diseases will be compared. In this stu...

OBJECTIVES β-Thalassaemia is widely found in Southwestern China. Characterisation of β-thalassaemia can improve screening and prenatal diagnosis for at-risk populations. DESIGN A retrospective study. METHODS In this study, the levels of haemoglobin alpha 2 (HbA2) and haemoglobin alpha (HbA) were analysed by gender for a total of 15 067 subjects screened by capillary electrophoresis. The cut...

Apart from few early biophysical studies, the relative thermal instability of HbE has been only shown by clinical investigations. We have compared in vitro thermal stability of HbE with HbA2 and HbA using optical spectroscopy. From absorption measurements in the soret region, synchronous fluorescence spectroscopy and dynamic light scattering experiments, we have found thermal stability of the t...

217414_x_at HBA2 3040 hemoglobin, alpha 2 hemoglobin complex [SUMMARY:] The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes the following five loci: 5'zeta pseudozeta pseudoalpha-1 alpha-2 alpha-1 -3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but the...

BACKGROUND The present study is designed to evaluate the reliability and cost effectiveness of cellulose acetate Hb electrophoresis and high performance liquid chromatography (HPLC) in the determination of HbA2 levels. METHODS The test population comprised 160 individuals divided into four groups: normal individuals, β-thalassemia trait (BTT) patients, iron deficiency anemia (IDA) patients, a...

Mutations in HBA1 and HBA2 genes cause α-thalassemia. Mutation in these two genes may produce deficient α subunit chains in hemoglobin in the red blood cells, and lead to anemia or even death. Since I was suspect of thalassemia, the objective of this study was to identify if there is any mutation in HBA1 and HBA2 genes in my genomic DNA. Using primers specific to the HBA1 and HBA2 genes, these ...

As a de facto reference laboratory, we receive samples from most parts of the country for hemoglobinopathy evaluation. These may be days or even weeks old before they reach our due to mail transport as well handling and analysis in other laboratories. We cannot control conditions storage arrive it is important evaluation know how these factors affect results relevant analyses. have previously i...

We evaluated the combination of two indices of red blood cell count (RBC) and red blood cell distribution with index (RDWI) and extrapolated HbA2 values in differential diagnosis of iron deficiency anemia (IDA) and beta-thalassemia traits (BTT) associated with IDA. A total 129 subjects were included in this study. RBC/RDWI was able to diagnose 32% beta-thalassemia with iron deficiency while 66%...

Increased HbA2 is a characteristic finding in minor beta thalassemia. Minor β-thalassemia is a heterozygote form of β-thalassemia that carries thalassemia genes but does not cause thalassemia disease. Diagnosis of carriers is done by CBC, RBC Index, and HbA2 test. Very few cases of people with minorthalassemia have a normal HbA2. According to the results of this pilot study it seams that percen...

We report a novel mutation at codon 24 of the α2-globin gene (HBA2: c.75T > A) found in a Sundanese family. This novel mutation was detected during prenatal diagnosis. The couple already had a 7-year-old boy who exhibited clinically severe α-thalassemia intermedia (α-TI), and he was found to be a compound heterozygote for the novel mutation at codon 24 and the previously described Hb Adana (HBA...