objective: the use of hema as a biocompatible material in dentin bonding systems and its potential for clinical applications has been well established. excess water can affect conversion of bonding resins. the aim of this study was to survey the effect of water on the degree of conversion of hema by fourier transform infra-red spectroscopy (ft-ir). materials and methods: in this experimental st...

BACKGROUND The resin monomer 2-hydroxyethyl methacrylate (HEMA) is known to be more cytotoxic than methyl methacrylate (MMA). Using a luciferase reporter assay system, we previously showed that MMA activates the glutathione S-transferase alpha 1 gene (Gsta1) promoter through the anti-oxidant responsive element (ARE). However, it is not known whether HEMA induces ARE-mediated transcription. ME...

One of the major components of dental polymerized resin-based restorative materials is 2-hydroxyethyl methacrylate (HEMA) and its release in monomeric form interferes with the oral cavity environment. This study aimed to evaluate HEMA monomeric effects on the co-culture of Streptococcus mitis and human gingival fibroblasts (HGFs). Streptococcus mitis DS12 and S. mitis ATCC 6249 were co-cultivat...

Copolymerization of 2-hydroxyethylmethacrylate (HEMA) with glycidylmethacrylate (GMA) in the presence of α-α'-azoisobisbutyronitrile (AIBN) resulted in the formation of hydrogel films carrying reactive epoxy groups. Thirteen kinds of different molecules with pendant NH2 group were used for modifications of the p(HEMA-GMA) films. The NH2 group served as anchor binding site for immobilization of ...

For the purpose of increasing the permeability to oxygen and equilibrium water content in contact lenses, polar copolymers with nonionic monomers such as 2-hydroxyethle methacrylate(HEMA) Is used. By adding the polar monomers to (HEMA), the permeability to oxygen and equilibrium with water content is increased and also the absorption of proteins on lens surface is changed. In this research, the...

The polymerization of 1-3 M 2-hydroxyethyl methacrylate (HEMA) initiated by riboflavin/triethanolamine system has been studied in the pH range 6.0-9.0. An approximate measure of the kinetics of the reaction during the initial stages (~5% HEMA conversion) has been made to avoid the effect of any variations in the volume of the medium. The concentration of HEMA in polymerized solutions has been d...

The HemA enzyme (glutamyl-tRNA reductase) catalyzes the first committed step in heme biosynthesis in the enteric bacteria. HemA is mainly regulated by conditional protein stability; it is stable and, consequently, more abundant in heme-limited cells but unstable and less abundant in normally growing cells. Both the Lon and ClpAP energy-dependent proteases contribute to HemA turnover in vivo. He...

Poly(HEMA) cryogel disks were synthesized by free radical polymerization of 2-hydroxyethylmethacrylate (HEMA), and then Cibacron Blue F3GA (CB), Reactive Green 19 (RG) and Congo Red (CR) were immobilized as dye ligands. Disks were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and swelling degree, porosity calculations. Cd2+ adsorption experi...

A series of polymers and copolymers of 2-hydroxyethyl methacrylate (HEMA) and methyl methacrylate (MMA) were synthesized in order to find surfaces that would adsorb minimal amounts of protein. The adsorption of albumin, lysozyme and immunoglobulin G from a three-way mixture of these proteins in isotonic buffered saline to the polymers was measured using 125I-labeled proteins. Apparently high pr...

Gene transfer of a factor VIII (FVIII) plasmid into hemophilia A (HemA) mice achieved supraphysiologic FVIII expression, but triggered production of high-titer FVIII-specific antibodies and loss of functional FVIII activity. To test whether FVIII-specific regulatory T cells (Tregs) can modulate immune responses against FVIII, we developed a HemA mouse model in which all T cells overexpressed Fo...