PURPOSE To determine the feasibility and accuracy of spectral-domain optical coherence tomography (SD-OCT) based grading of anterior chamber cell, using aqueous sampling as a standard, in a rabbit model of anterior uveitis. METHODS Adult Dutch-belted rabbits were preimmunized with M. tuberculosis (Tb) H37RA antigen, 1 week prior to induction of anterior uveitis with an intracameral injection ...

One of the traditional methods of cell viability analysis is the use of trypan blue dye exclusion staining. This technique has been the standard methodology used in academic research laboratories and industrial biotechnology plants. Cells were routinely counted manually with a hemocytometer. In recent years, modern automated instrumentation has been introduced to supplement this traditional tec...

VARIABILITY IN THE PRODUCTION OF FUNGAL SPORES AND IN THE MEASUREMENT OF SPORE YIELDS WAS INVESTIGATED IN FOUR SPECIES OF FUNGI: Colletotrichum gloeosporioides, Colletotrichum coccodes, Colletotrichum phomoides, and Acremonium strictum. When the fungi were grown on solid medium in microplates and spore yields were measured by counting the subsamples with a hemacytometer, the variability among h...

A suspension of Ketawaspreparedby trypsinization of stock cultures in Blake bottles. After determining the cell count with a hemocytometer the suspension was diluted with medium to give a c@int of' 75,000 cells per 0. 9 ml. Using an automatic pi pette, 0. 9 m:1@. of the Hela suspension was delivered into each of the cups of the plastic panel and the test compound added in 0.1 ml. saline or in 0...

Total count and differential count of leukocytes or white blood cells (WBC) in blood samples are very important pathological factors for diagnosing a disease. There are not enough pathological infrastructures in the remote places of India and other developing countries. The objective of this work is to design a system, compatible with telemedicine, for automatic calculation of the total count a...

Chlorella vulgaris had the ability to accumulate heavy metals in their bodies, so they could be used as biosorbent handling metal pollution waters. The effectiveness of C. remediation cadmium (Cd) would tested this present study. were cultured for 14 days photobioreactor which was an enclosed chamber that fully aerated and illuminated with LED lamps. Cadmium concentrations 0 (control), 0.05, 0....

Peripheral human corneal epithelium grows better in tissue culture than central epithelium, but it is not known whether ocular limbal epithelium grows even better than does the peripheral corneal epithelium. In this work we compared the growth kinetics of limbal and peripheral human corneal epithelial cells in tissue culture. Four 1-2 mm2 explants, removed from the limbus or from peripheral cor...

A method has been developed by which the density and weight of an individual yeast cell can be measured with a reasonable degree of accuracy. The average weight of yeast cells in a cake is rather easily-determined by weighing out a gram of yeast and counting the number of cells with a hemacytometer, but measuring the weight of an individual cell presents a different problem, the solution of whi...