Rabbit antibodies specific for the major tadpole and frog hemoglobin components of R. catesbeiana were used for the detection of the two hemoglobins inside single cells . The antisera, after fractionation by ammonium sulfate precipitation and diethylaminoethyl (DEAE)cellulose chromatography, were conjugated with fluorescein isothiocyanate for the antifrog hemoglobin serum and tetramethylrhodami...

We demonstrate that 5,5'-dithiobis(2-nitrobenzoate) - DTNB - reacts with only CysF9[93]beta and CysB5[23]beta among the multiple sulfhydryl groups of the major and minor hemoglobins of the Japanese quail (Cortunix cortunix japonica). K(equ), the equilibrium constant for the reaction, does not differ very significantly between the two hemoglobins. It decreases 430-fold between pH approximately 5...

A boronate affinity column method for the measurement of glycosylated hemoglobins was evaluated. In the procedure the glycosylated hemoglobins were bound by immobilized boronic acid to separate them from nonglycosylated hemoglobins. Elution of bound glycosylated hemoglobins was carried out with sorbitol buffer, and the absorbance was read at 414 nm. The method was linear to a glycosylated hemog...

A B S T R A C T The extent of dissociation of various hemoglobins into subunits was estimated from their elution volumes (V.) on G-100 Sephadex. Under the same controlled conditions carboxyhemoglobins A, A3 (A,), F, S, and C all had the same elution volumes. The carboxy and cyanmet derivatives of hemoglobin Kansas (a variant with very low oxygen affinity) had a relatively high Ve, indicating a ...

A large amount of data is currently available on the adaptive mechanisms of polar bony fish hemoglobins, but structural information on those of cartilaginous species is scarce. This study presents the first characterisation of the hemoglobin system of one of the longest-living vertebrate species (392 ± 120 years), the Arctic shark Somniosus microcephalus. Three major hemoglobins are found in it...

Macaca nemestrina have two fetal hemoglobins which differ in the structures of their .y chains. Thus animals of this species, like man, appear to possess duplicate y-chain loci. In this study, the ontogenesis of hemoglobins in M. nemestrina was followed in animals ranging in age from the 60th day of gestation to adulthood. Fetuses in the 60th day of gestation had the two fetal hemoglobins and a...

The oxygenation of hemoglobins is accompanied by the dissociation of protons. The number of protons discharged is inversely related to the size of the mammal from which the hemoglobin comes. The number of mercuric ions which are immediately bound by hemoglobins is approximately equal to the number of protons dissociated during oxygenation. Pretreatment of human hemoglobin by N-ethylmaleimide, w...

The /3 chain amino terminus of one or more hemoglobins in the blood of members of the Felidae family is substituted with an acetyl group. Such acetylated hemoglobins occur together with nonacetylated components in variable proportions in blood of d8erent members of the family. In the lion, tiger, and snow leopard, the acetylated component comprises about 90% of the total hemoglobin; in the serv...

The larval hemoglobins of the insect Chironomus fenfans, present in solution in hemolymph, were demonstrated to be exclusively monomeric with a molecular weight of approximately 15,900. This finding was based on studies of sedimentation velocity at various concentrations and with the use of agents generally effective in subunit separation, together with determinations by sedimentation equilibri...

The identification of specific antibodies for a variety of inherited hemoglobin variants presents the opportunity to develop sensitive immunochemical measurement techniques for such variants. In this study antibodies specific for human hemoglobins A1 and S were isolated from immune rabbit serum. These were labeled with either fluoroscein isothiocyanate or tetramethylrhodamine isothiocyanate. Er...