The effects of cAMP-analogue dibutyryl-cAMP and anticalmodulin W-7 were studied on de novo synthesis and secretion of lipids in cultures of hepatoma McArdle RH7777 cells and normal rat hepatocytes. Dibutyryl-cAMP and W -7 separately caused a significant decrease in the secretion of de novo synthesized triacyl [3H]glycerol in both cultures of McArdle cells and rat hepatocytes. The inhibito...

high levels of regulated oncogen-alpha (gro-a) expression have been observed in the liver. gro-a stimulates proliferation of epithelial cells and induction of rolling and extravascular migration of neutrophils and mononuclear cells. given the above observations, this chemokine was chosen to be analyzed in freshly isolated and cultured hepatocytes. in this study, hepatocytes (2×106 cell/ml) were...

conclusions our results suggest that h. parva and h. oculata may be promising therapeutic candidates for the treatment of hcc, following further confirmatory in vivo experiments and clinical trials. results our results showed that h. parva methanolic extracts (250, 500, and 1000 µg/ml) and h. oculata methanolic extracts (200, 400, and 800 µg/ml) increased reactive oxygen species (ros) formation...

Alkaloids of the aerial parts of chelidonium were extracted in the salt form, and their aqueous solutions were prepared in different concentrations. Rat hepatocytes were obtained by liver perfusion. The alkaloidal solutions were added to suspensions of hepatocytes in petri dishes and the mixtures were incubated. Two types of controls have been used in one type. no alkaloidal extract was ad...

alkaloids of the aerial parts of chelidonium were extracted in the salt form, and their aqueous solutions were prepared in different concentrations. rat hepatocytes were obtained by liver perfusion. the alkaloidal solutions were added to suspensions of hepatocytes in petri dishes and the mixtures were incubated. two types of controls have been used in one type. no alkaloidal extract was added t...

ATP binding cassette transporter A1 (ABCA1) limits the formation of high density lipoproteins (HDL) as genetic loss ABCA1 function causes virtual HDL deficiency in patients with Tangier disease. Mice a hepatocyte-specific knockout (Abca1 HSKO) have 20% wild type (WT) plasma HDL-cholesterol levels, suggesting major contribution hepatic to phenotype. Whether sphingolipids are reduced disease and ...

The isolation of primary hepatocytes from liver tissue farm animals yields a very high number cells, and part them can be stored by cryopreservation for future experiments. As no experience exists with the cattle, our study aimed at bovine use different protocols compared pig. We tested freezing media (William’s Medium E vs. University Wisconsin solution), cryoprotectants (dimethyl sulfoxide wi...

Modulatory effects of l-carnitine, acetate, propionate, and 5-tetradecyloxy-2-furoic acid (TOFA; an inhibitor acetyl-CoA carboxylase) on oxidation esterification [1-14C]-palmitate were studied in hepatocytes isolated from phlorizin-treated control wethers. Our hypotheses that (1) palmitate would be greater sheep injected with phlorizin; (2) l-carnitine increase more (3) acetate propionate decre...

Objective – Our study aimed at evaluating changes in liver tissues after simulated injury and use of novel hemostatic agents (containing tranexamic acid) the course vivo experiment. Material Methods The following were studies: TachoComb collagen plate (Group 1); samples local based on sodium carboxymethyl cellulose (CMC), Na-CMC 2); + acid 3). We performed 30 rabbits with laceration via laparos...