نتایج جستجو برای: hepg2 cell line
تعداد نتایج: 1973091 فیلتر نتایج به سال:
Introduction: Antibiotic resistance has been reported as one of the world's most critical public health problems. Recent investigations have demonstrated that venom of some species of snakes have antimicrobial and anticancer activities. In this study, we investigated the antibacterial and anticancer effects of Persian horned viper venom. Antibacterial activity was examined on Escherichia coli,...
objective: understanding gene expression variations by using rna transcript analysis methods during hepatic viral protein interactions with the ifn pathway in hepatic cell lines has recently gained importance. one of the most powerful techniques in gene expression quantification is quantitative real-time rt-pcr. reference genes used as normalizer in this method may be affected across various ex...
Introduction: Cell culture is a process in which the cells ware isolated from original tissue, dispersed in liquid media and then placed in culture plate where the cells adhere together and propagate. Today, this method is used for assessment of cell toxicity, its mechanisms and effect of different compounds on intracellular components. Methods: Clonogenic assay was used for assessment of cel...
The Effect of Valproic Acid Drug on the Expression SOCS1, SOCS3, JAK1, JAK2, STAT3, STAT5A and STAT5B Genes in HepG2 Cell Line Liver Cancer
methods the current study generated two recombinant lentiviruses, plex-gcn and plex-gcp, bearing noxa and puma, respectively. transduction of both genes to hepatocarcinoma (hepg2) was verified using fluorescent microscopic analysis, western blotting, and quantitative real-time polymerase chain reaction (pcr). to evaluate the potential of noxa and puma to initiate apoptosis, a caspase-9 real-tim...
Cytokines protein or glycoproteins synthesized by a wide range of cell types including lymphocytes, macrophages, platelets, and monocytes. Cytokines induse synthesis of acute phase protein in hepatocytes. So far eight cytokines has been shown can induce acute phase protein synthesis in hepatocyte and hepatoma cell culture. There are evidences that transforming growth factor-alpha (TGF-a) secr...
background hepatocytes are used as an in vitro model to evaluate drug metabolism. human hepatocyte transplant has been considered as the temporary treatment of acute liver failure. optimization freezing methods is very important to preserve both cell viability and function which are achieved by cryopreservation mostly always. objectives the present study aimed to investigate the cryoprotective ...
objective(s): protective effects of different extracts and essential oil from pimpinella anisum l. seeds were examined against carbon tetrachloride (ccl4)-induced toxicity. the parameters such as serum transaminases, lactate dehydrogenase activity, hepatic glutathione content, liver lipid peroxidation and histopathological changes of liver were assessed as toxicity markers. in the in...
methanolic extract of 15 iranian medicinal plants were prepared and tested for their cytotoxic activities against three cancer cell lines (mcf7, hepg2 , wehi164) and one normal cell line (mdbk). some plants showed cytotoxic activities. the extract of ferula szowitsiana root, which proved to be the most active, was chosen for further phytochemical studies. the major compounds of the most potent ...
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