conclusions the obtained results indicate that rsm is an effective method for the optimization of culture conditions for hes gene expression in e. coli. results a maximum rhes mrna level of 376.16% was obtained under the following conditions: 0.6 mm iptg, 7 hours post-induction time, and 0.9 cell density before induction. the level of rhes mrna was significantly correlated with post-induction t...

  In this paper, we establish exact solutions for the time-fractional Klein-Gordon equation, and the time-fractional Hirota-Satsuma coupled KdV system. The He’s semi-inverse and the Kudryashov methods are used to construct exact solutions of these equations. We apply He’s semi-inverse method to establish a variational theory for the time-fractional Klein-Gordon equation, and the time-fractiona...

We introduce the concept of sandwich set of two fuzzy matrices.By using the new concept of sandwich set, we provide a method for finding a semi inverse of max-min product of fuzzy matrices. 1. Introduction Generalized inverse of matrices and its applications are studied by Rao and Mitra[3]. Ren et.al[4] introduced the concept of sandwich sets of matrices. By using the sandwich sets of matrices,...

A system of semi-discrete coupled nonlinear Schrödinger equations is studied. To show the complete integrability of the model with multiple components, we extend the discrete version of the inverse scattering method for the single-component discrete nonlinear Schrödinger equation proposed by Ablowitz and Ladik. By means of the extension, the initial-value problem of the model is solved. Further...

This article concernes the method of approximate inverse to solve semi-discrete, linear operator equations in Banach spaces. Semi-discrete means that we search a solution in an infinite dimensional Banach space having only a finite number of data available. In this sense the situation is applicalble to a large variety of applications where a measurement process delivers a discretization of an i...

This chapter describes the methods we use to maintain and expand undifferentiated human embryonic stem (hES) cells on human and mouse feeder cells. All of the available hES cells have been derived and propagated on primary mouse embryonic fibroblasts as feeder cells that have been mitotically inactivated. We found that hES cells can be successfully cultured on selected human feeder cells, such ...

Human embryonic stem (hES) cells hold great therapeutic potential for cell transplantation. To date, it remains uncertain whether undifferentiated hES cells can differentiate into cardiac lineage in vivo during myocardial infarction. Here we provide the first report that undifferentiated hES cells can survive in rat hearts during myocardial infarction without the formation of teratoma using und...

Due to widespread applications of human embryonic stem (hES) cells, it is essential to establish effective protocols for cryopreservation and subsequent culture of hES cells to improve cell recovery. We have developed a new protocol for cryopreservation of dissociated hES cells and subsequent culture. We examined the effects of new formula of freezing solution containing 7.5% dimethylsulfoxide ...