objective: classic cell culture and karyotyping is routinely used for prenatal detection of different chromosomal abnormalities. molecular cytogenetic techniques have also recently been developed and used for this purpose. quantitative florescence pcr using short tandem repeat (str) markers has more potential for high throughput diagnosis. marker heterozygosity in short tandem repeats (str) is ...

Heterozygosity-fitness correlations use molecular measures of heterozygosity as proxy estimates of individual inbreeding coefficients (f) to examine relationships between inbreeding and fitness traits. Heterozygosity-fitness correlations partly depend on the assumption that individual heterozygosity and f are strongly and negatively correlated. Although theory predicts that this relationship wi...

Triploid molluscs grow significantly faster than diploids in most species studied so far, a phenomenon that has been referred to as triploid gigantism. Three hypotheses have been proposed, attributing triploid gigantism to sterility, increased heterozygosity, or cell size. Testing the heterozygosity hypothesis, the authors measured the body size and allozyme heterozygosity in Ž . Ž . three repl...

Studies in a multitude of taxa have described a correlation between heterozygosity and fitness and usually conclude that this is evidence for inbreeding depression. Here, we have used multilocus heterozygosity (MLH) estimates from 15 microsatellite markers to show evidence of heterozygosity-fitness correlations (HFCs) in a long-distance migratory bird, the light-bellied Brent goose. We found si...

this study aimed to estimate microsatellite based genetic diversity in two lines (the selected rirs and control line rirc) of rhode island red (rir) chicken. genomic dna of 24 randomly selected birds maintained at central avian research institute (india) and 24 microsatellite markers were used. microsatellite alleles were determined on 6% urea-page, recorded using geldoc system and the samples ...

cystic fibrosis (cf) is a life-limiting autosomal recessive disorder affecting principally respiratory and digestive system . it is caused by cystic fibrosis transmembrane conductance regulator (cftr) gene mutation. the aim of this study was to determine the extent of repeat numbers and the degree of heterozygosity for c.3499+200ta(7_56) and d7s523 located in intron 17b and 1 cm proximal to the...

The extent of inbreeding depression and the magnitude of heterozygosity-fitness correlations (HFC) have been suggested to depend on the environmental context in which they are assayed, but little evidence is available for wild populations. We combine extensive molecular and capture-mark-recapture data from a blue tit (Cyanistes caeruleus) population to (1) analyze the relationship between heter...

The general hypothesis of mate choice based on non-additive genetic traits suggests that individuals would gain important benefits by choosing genetically dissimilar mates (compatible mate hypothesis) and/or more heterozygous mates (heterozygous mate hypothesis). In this study, we test these hypotheses in a socially monogamous bird, the blue tit (Cyanistes caeruleus). We found no evidence for a...

Two commercial broiler lines were used in an experiment to map quantitative trait loci (QTL) affecting disease resistance. Chickens from these lines were genotyped with 27 microsatellite markers to estimate heterozygosity and polymorphism information content (PIC), the probability that one parent is heterozygous at a marker locus and the other has a different genotype. Heterozygosity estimated ...

population mean heterozygosity (i.e. 12 data points). Both analyses were statistically significant, indicating that populations with low heterozygosity have relatively abnormal sperm. Two alternatives to inbreeding depression are consistent with this result: a third, unknown factor causes some populations to have low heterozygosity and relatively abnormal sperm; for instance, environmental hete...