separation of ten sedative- hypnotic drugs was performed by rp-hplc using mixed micellar mobile phase. effect of temperature, type and amount of organic modifier in mobile phase on efficiency (n) and asymmetry factor (b/a) showed that, the appropriate conditions for a good separation were 35°c and 7% (v/v) butanol in mobile phase.  variations of selectivity factor versus butanol concentration, ...

conclusions this method could be feasibly used for purification of large quantities of allicin from garlic extract. results semi-preparative hplc yielded a distinct peak with a retention time of 12.28 minutes. the purity (≥ 95%) and biological activity (p > 0.05) of purified allicin were comparable to the standard allicin. purified allicin produced same peak areas compared to the standard allic...

in the present study, a comprehensive and systematic strategy was described to evaluate the performance of several three-way calibration methods on a bio-analytical problem. parallel factor analysis (parafac), alternating trilinear decomposition (atld), self-weighted alternating trilinear decomposition (swatld), alternating penalty trilinear decomposition (aptld) and unfolded partial least squa...