Salmonella enterica serovar Typhimurium causes self-limiting gastroenteritis in humans and a typhoid-like disease in mice that serves as a model for typhoid infections in humans. A critical step in Salmonella pathogenesis is the invasion of enterocytes and M cells of the small intestine via expression of a type III secretion system, encoded on Salmonella pathogenicity island 1 (SPI-1), that sec...

Hila Hashemi,1 A. Oskooi,2 J. D. Joannopoulos,3 and Steven G. Johnson1 1Department of Mathematics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA 2Department of Electronic Science and Engineering, Kyoto University, Japan 3Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA (Received 20 February 2011; published 11 August 2...

Salmonella typhimurium invasion of nonphagocytic cells requires the expression of a type III secretion system (TTSS) encoded within Salmonella pathogenicity island 1 (SPI1). TTSS gene transcription is activated in response to environmental signals and requires transcriptional regulators encoded within (HilA) and outside (SirA) SPI1. Two unique loci, sirB and sirC, which contribute to SPI1 gene ...

An early step in the pathogenesis of Salmonella enterica serovar Typhimurium infection is bacterial penetration of the intestinal epithelium. Penetration requires the expression of invasion genes found in Salmonella pathogenicity island 1 (SPI1). These genes are controlled in a complex manner by regulators in SPI1, including HilA and InvF, and those outside SPI1, such as two-component regulator...

DNA regulatory motifs reflect the direct transcriptional interactions between regulators and their target genes and contain important information regarding transcriptional networks. In silico motif detection strategies search for DNA patterns that are present more frequently in a set of related sequences than in a set of unrelated sequences. Related sequences could be genes that are coexpressed...

background: several regulatory proteins are involved in salmonella invasion. the key regulator of spi-1 (salmonella pathogenicity island 1 ) is hila, a transcriptional activator encoded on spi-1 that regulates the expression of the spi-1 secretion system. objectives: importance of hila mutation on s. enteritidis colonization and shedding in layer hens was evaluated in a long-term experiment. me...

An aspect-oriented modeling approach to the Car Crash Crisis Management System (CCCMS) using the High-Level Aspect Language (HiLA) is described. HiLA is a language for expressing aspects for UML static structures and UML state machines. In particular, HiLA supports both a static graph transformational and a dynamic approach of applying aspects. Furthermore, it facilitates methodologically turni...

background : salmonella actively stimulates its own uptake into the epithelial cells by inducing cytoskeleton rearrangements and membrane ruffling triggered by some proteins secreted by salmonella into the cytosol of the epithelial cells via a type iii secretion system (ttss) encoded by genes of the salmonella pathogenicity island 1 (spi-1). hila is a transcriptional activator encoded on salmon...

UNLABELLED The transcription factors HilA and SsrB activate expression of two type III secretion systems (T3SSs) and cognate effectors that reprogram host cell functions to benefit infecting Salmonella in the host. These transcription factors, the secretion systems, and the effectors are all encoded by horizontally acquired genes. Using quantitative proteomics, we quantified the abundance of 2,...

Salmonella enterica invades the intestinal epithelium of the host using a type III secretion system encoded on Salmonella pathogenicity island 1 (SPI1). The bacteria integrate environmental signals from a variety of global regulatory systems to precisely induce transcription of SPI1. The regulatory circuit converges on expression of HilA, which directly regulates transcription of the SPI1 appar...