We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

In ecosystems with frequent surface fires, fire and fuel heterogeneity at relevant scales have been largely ignored. This could be because complete burns give an impression of homogeneity, or due to the difficulty in capturing fine-scale variation in fuel characteristics and fire behaviour. Fire movement between patches of fuel can have implications for modelling fire spread and understanding e...

garshasp has peregrinated an agelong path from avesta epoch to the time of preparation of old historical context and iran’s national epic. garshasp, who is the unique hero of iran’s old mythology, that his brave acts, such as killing the dragon and ogre lobbing are important parts of the heroic themes included in the avesta, through the advent of pahlavi literary context because of reasons such...

For over a century the Mexican state has justified its control of forests by claiming that rural people are ignorant and destructive fire setters, in the face of abundant evidence to the contrary. Academic and popular stereotypes of the state have tended to assume that official power and knowledge go hand in hand. In an institutional ethnography of the Mexican environment agency, SEMARNAP, I sh...

We use a powerful extension of the classical method heat potentials, recently developed by present author and his collaborators, to solve several significant problems financial mathematics. consider following in detail: (A) calibrating default boundary structural framework constant intensity; (B) calculating probability for representative bank mean-field framework; (C) finding hitting time dens...