Homologous recombination is essential for the accurate repair of double-stranded DNA breaks (DSBs). Initially, RecBCD complex resects ends DSB into 3′ single-stranded on which a RecA filament assembles. Next, locates homologous template sister chromosome. Here we directly visualize DSBs in single cells, using high-throughput microfluidics and fluorescence microscopy. We find that, Escherichia c...

There are hypotheses in the history of human culture and civilization in which we can find undeniable similarities and commonalities among them, despite their vastly different cultural and historical contexts. For example, finding commonalities between the language of two artistic aesthetic hypotheses, Sufism and Surrealism, which are very different from each other in terms of context, time as ...

DNA damage, in the form of DNA double-strand breaks, poses a considerable threat to genomic integrity and cell survival. If left unrepaired, a single double-strand break is sufficient to cause cell death and, if repaired inappropriately, a double-strand break may give rise to a potentially oncogenic translocation. Double-strand breaks in genomic DNA may arise accidentally in a number of ways, i...

In this paper, we examine the properties of radial distance which has been used as a tool to compare shape simple surfacic objects. We give rigorous definition and derive its theoretical properties, in particular under conditions it satisfies properties. show how computation can be implemented practice made faster by use an analytical formula Fast Fourier Transform. Finally, conduct experiments...

An inactivated trivalent fowl cholera vaccine consisted of serotypes 1, 3 and 4 Pasteurella multocida strains was prepared. The vaccine provided 70-100% protection against challenge with homologous starins. ELISA assay showed a considerable increase in antibody titer after towice vaccination of 8 weeks chickens. It was found that the trivalent vaccine can induce immunogenic response in vaccinat...

introduction: listeria monosytogenes is a gram-positive organism, frequently found in the environment and is responsible for serious food-born diseases such as perinatal infections, septicaemia and meningoencephalitis in humans and animals. materials & methods: distribution of the ctpa determinant among l. monosytogenes isolated from environmental and clinical samples was investigated using pcr...