hop stunt viroid (hsvd) isolates have been reported as the causal agent of citrus cachexia in mazandaran province and recently shown to be associated with yellow corky vein disease of sweet orange and split bark disorder of sweet lime in the fars province, iran. in the present work isolation and partial characterization of viroids from citrus trees affected by gummy stem blight is reported from...

plant samples were collected from yazd, isfahan, fars and tehran provinces and examined for their possible infection by hop stunt viroid (hsvd). the experimental host range of the viroid was determined by injection of infected sap into greenhouse grown seedlings and subsequent assay of inoculated plants by rt-pcr. fig, mulberry and citrus isolates of the viroid were compared with respect to hos...

Figure 1 Figure 2 Citrus fruits are traditional agricultural products in Egypt. Citrus volkameriana is the best rootstock for growing citrus in Egypt’s desert areas (newly reclaimed land). It is far superior to other rootstocks used in Egypt in its resistance to Citrus tristeza virus as well as its tolerance to drought. Constraints to fruit production are mainly related to tree decline caused b...

apple scar skin disease (assd) is one of the destructive diseases of pome fruits which is caused by assvd. we report molecular detection of assvd in symptomatic apples cv. red delicious with simultaneous presence of a new variant of hsvd from khorasan razavi province. this is the first report on the association of hsvd with assvd in apple trees showing merely symptoms of apple dapple on the fruit.

background and aims: plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. reverse transcriptase-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certification of plant propagating materials. the main object of the present study was the optimization ...

Citrus Bent Leaf Viroid (CBLVd) and Dwarfing (CDVd), from genus Apscaviroid, are two of the widely distributed citrus viroids among eight reported to date. They have been found induce reduction in canopy volume fruit production trees on trifoliate orange hybrids rootstocks. Moreover, infected only with CBLVd or combinations Exocortis (CEVd), Hop Stunt (HSVd) CDVd associated a poor development r...

Background and Aims: Plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. Reverse transcriptase-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certification of plant propagating materials. The main object of the present study was the optimization ...

Hop stunt viroid RNA (HSV) has been detected by molecular hybridization in stunted plants of the Japanese cultivar Kirin II cultivated in Korea (1,2). For propagation, the viroid was transmitted to the wild curcarbU Benincasa Mspida which developed HSV-spedfic severe growth retardation. Isolation, purification, reverse transcription of the viroid-RNA with aid of viroid-specific primers, and seq...