A review of indirect photometric and fluorometric detection in high-performance liquid chromatography (HPLC) is presented. A functional definition of indirect detection, somewhat broader and simpler than what was often used to characterize these techniques, was employed for this review. Emphasis was placed on distinguishing different approaches to indirect photometric and fluorometric detection...

BACKGROUND Central Middlesex Hospital, in northwest London, has screened neonates for hemoglobinopathies, using the established manual technique of isoelectric focusing (IEF) since 1989. Recently, this laboratory has faced a large increase in the number of samples tested per year. This study compared the detection of hemoglobin abnormalities between the existing manual IEF method and that of au...

In this article, we describe a fast and specific method to measure 5FU with HPLC tandem-mass spectrometry. Reversed-phase HPLC was combined with electrospray ionization tandem mass spectrometry and detection was performed by multiple-reaction monitoring. Stable-isotope-labeled 5FU (1,3-15N2-5FU) was used as an internal standard. 5FU was measured within a single analytical run of 16 min with a l...

As one of the widely used veterinary macrolide antibiotics, the environmental risks of tylosin (TYL) received more and more attention. The purpose of the present study was to develop a modified, rapid and precise RP-HPLC method with PDA detection for the determination of the macrolide antibiotic tylosin in liquid samples. The proposed two HPLC methods offered short retention time (< 6.8 min), h...

A sensitive and specific high-performance liquid chromatography (HPLC) method based upon laser-based polarimetric detection is developed for the determination of six tetracycline analogues. By interfacing the laser-based polarimeter online with an HPLC system, the specific rotation of each analogue is obtained as compounds elute from the separation system. The six structurally similar tetracycl...

We present a candidate Reference Method for determining the concentration of serum creatinine. The method is based on HPLC combined with enzymatic determination. Creatinine plus 14[C]creatinine is extracted by cation-exchange chromatography, subjected to reversed-phase HPLC, and finally quantified enzymatically. Enzymatic measurement ensures no interference from co-eluting compounds, which has ...

Analysis of fructose and glucose from glucose isomerization process using immobilised glucose isomerase (IGI), {Sweetzyme from Novozymes} are often performed by HPLC methods with refractive index (RI) detector. This study is focused on developing new methods of measuring glucose and fructose using a specific carbohydrate column. The importance of this research, primarily based on the performanc...

Rape pollen has always been considered as a research hotspot in health foods and pharmaceuticals due to its abundance of natural active ingredients. In this work, compound with antioxidant activity was directly isolated from the methanol extract rape using two-step procedure, under supervision online HPLC−1,1-diphenyl-2-picrylhydrazyl (HPLC-DPPH) detection. Firstly, HPLC−DPPH detection used ide...