As the primary refractive surface of the eye, clarity of the cornea is essential for optimal visual acuity. Many disease processes can irreversibly disrupt corneal clarity requiring corneal transplantation to restore visual function. The most common indication for corneal transplantation is opacification from corneal edema. Corneal edema arises from dysregulation of fluid homeostasis in the cor...

OBJECTIVE To evaluate the effects of phacoemulsification energy on the redox state and mitochondrial distribution of cultured human endothelial cells. METHODS Human corneal endothelial cells from fresh banked human donor tissue not suitable for transplantation were harvested and cultured. Cellular autofluorescence images were obtained using an inverted microscope. The redox fluorometric ratio...

The native intermediate filament network within human corneal endothelium was identified by a monoclonal antibody to vimentin intermediate filament protein. Human corneal endothelial cells in tissue culture were shown to react positively to this monoclonal antibody by indirect immunofluorescence microscopy. In cryostat sections of human cornea, only the endothelial cells and keratocytes stained...

Currently there are limited treatment options for corneal blindness caused by dysfunctional corneal endothelial cells. The primary treatment involves transplantation of healthy donor human corneal endothelial cells, but a global shortage of donor corneas necessitates other options. Conventional tissue approaches for corneal endothelial cells are based on EDTA-trypsin treatment and run the risk ...

Cell-based therapies have been increasingly explored as a potential alternative treatment method of corneal endothelial dysfunction. Up to date, researchers have mainly dedicated enormous efforts to establishing fundamental techniques for culture and characterization of human corneal endothelial cells (HCECs) and human corneal endothelial progenitor cells (HCEPCs). However, in terms of cell del...

PURPOSE To describe genes expressed in human corneal endothelial cells and identify novel genes. METHODS Sixteen human donor corneas that had no history of corneal disease, infection, or intraocular surgery were used within 7 days of death. Total RNA was extracted from corneal endothelial cells with attached Descemet membranes. A 3'-directed cDNA library was constructed from mRNA by using a p...

purpose: to evaluate the adverse effects of intravitreal injection of sterile chloramphenicol eye drops on corneal endothelial cells of donated corneas. methods: 100 donor corneas were enrolled in the study. one eye of each donor underwent intravitreal injection of sterile chloramphenicol eye drops following vitreous tap and the fellow eyes were served as controls. after corneo-scleral excision...

Current treatments for reversible blindness caused by corneal endothelial cell failure involve replacing the failed endothelium with donor tissue using a one donor-one recipient strategy. Due to the increasing pressure of a worldwide donor cornea shortage there has been considerable interest in developing alternative strategies to treat endothelial disorders using expanded cell replacement ther...

purpose: to evaluate corneal endothelial cells of donors who died from aluminum phosphide intoxication. methods: endothelial cell count and quality of eight corneas from four donors with aluminum phosphide intoxication were evaluated by slit lamp biomicroscopy and specular microscopy at the eye bank of i.r. iran. confocal scan examination was carried out only in one of the recipients. results: ...

background: angiogenesis, the development of new blood vessels, is an important process in tissue development and wound healing, but becomes pathologic when associated with solid tumor growth, proliferative retinopathies, and rheumatoid arthritis. accurate and reliable qualification of neovascular (angiogenic) response, both in vitro and in vivo, is an essential requirement for the study of new...