Purpose: To evaluate the influence of triamcinolone acetonide on the proliferation of retinal pigment epithelum cells in an experimental cell culture study. Methods: Pooled fresh porcine retinal pigment epithelium cell cultures were exposed to triamcinolone acetonide in increasing concentrations after the solvent agent had been removed. The proliferation rate was measured using the (3-(4,5-dime...

von Humboldt Foundation, Bonn, Germany. ABSTRACT Objective The pathogenesis of central serous chorioretinopathy is poorly understood. It is believed to be due to dysfunction of the retinal pigment epithelium and/or choroid, and has been associated with elevated levels of epinephrine and administration of corticosteroids. Epinephrine and corticosteroids have previously been shown to induce apopt...

aim: to report a patient with fundus flavimaculatus or macular dystrophy with flecks, who came to medical attention due to deteriorated vision in one eye and combined hamartoma of the retina and retinal pigment epithelium. case presentation: a 26 year old lady was referred with history of progressive decreased vision in both eyes since childhood with recently deteriorated vision in her right ey...

Retinal pigment epithelial dysfunction is believed to be the main cause of many debilitating retinal diseases of which age-related macular degeneration is the most common. In this disease, the retinal pigment epithelial dysfunction leads to photoreceptors damage causing severe vision loss. The retinal pigment epithelium and Bruch's membrane suffer cumulative damage over lifetime, which is thoug...

Retinoblastoma cells of the Y79 line were co-cultivated with human fibroblasts and bovine iris and retinal pigment epithelium in tissue culture. The Y79 cells, which characteristically grow as a suspension culture, were found to attach directly to the fibroblasts and pigment epithelium on the flask surface. Electron microscopic examination of the fibroblast-retinoblastoma co-cultures revealed n...

PURPOSE To determine whether curcumin induces expression of the defensive enzyme heme oxygenase-1 (HO-1) and protects cells against oxidative stress in cultured human retinal pigment epithelial cells. METHODS Effective concentrations and toxicities of curcumin were determined after 3 h of curcumin treatment with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Confluent...

The optic vesicle is a multipotential primordium of the retina, which becomes subdivided into the neural retina and retinal pigmented epithelium domains. Although the roles of several paracrine factors in patterning the optic vesicle have been studied extensively, little is known about cell-autonomous mechanisms that regulate coordinated cell morphogenesis and cytodifferentiation of the retinal...

Pigment epithelial cell membrane potentials were measured in an in vitro frog retina-pigment epithelium-choroid preparation. Light stimuli hyperpolarized the apical membrane of the pigment epithelium. This hyperpolarization was the source of the c-wave of the electroretinogram. Light stimuli also decreased retinal extracellular potassium ion concentration, [K+]O, with the same time course as th...

OBJECTIVE To determine the distribution of 2 intracellular oxygen-carrying molecules, neuroglobin (NGB) and cytoglobin (CYGB), in specific retinal cell types of human retinas. METHODS Specific antibodies against NGB and CYGB were used in immunohistochemical studies to examine their distribution patterns in human retinal sections. Double-labeling studies were performed with the anti-NGB and an...