BACKGROUND This study aimed to investigate the inhibitory effect of blueberry anthocyanin (BBA) on Angiotensin II (Ang II)-induced apoptosis of human umbilical vein endothelial cells (HUVECs), and its regulation mechanisms involving Bax and Caspase 3. MATERIAL AND METHODS HUVECs were first treated by different concentrations of Ang II (10-9, 10-8, 10-7, 10-6, 10-5, and 10-4 mol/L) and BBA (80, ...

Biomaterials with characteristics similar to extracellular matrix and suitable bioprinting properties are essential for vascular tissue engineering. In search biomaterials, this study investigated the three hydrogels alginate/hyaluronic acid/gelatin (Alg/HA/Gel), pre-crosslinked alginate di-aldehyde gelatin (ADA-GEL), methacryloyl (GelMA) respect their mechanical survival, migration, proliferat...

The adhesion of monocytes to human umbilical vein endothelial cells (HUVECs) plays a crucial role in the initiation of atherosclerosis. Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are two important molecules involved in the adhesion of monocytes to HUVECs. Previous studies have suggested that artemisinin, apart from an anti-malarial agent, also has ...

BACKGROUND Ischemia-reperfusion injury leads to the activation and endothelial deposition of complement. We investigated whether exposure of human umbilical vein endothelial cells (HUVECs) to hypoxia and/or reoxygenation activates complement and decreases HUVEC-surface expression of the C3 regulatory proteins CD46 and CD55. METHODS AND RESULTS HUVECs were subjected to 0, 12, or 24 hours of hy...

Cryopreservation is widely used in regenerative medicine for tissue preservation. In the present study, the effects of cryopreservation on excretory function, cellular adhesion molecules and vessel lumen formation in human umbilical vein endothelial cells (HUVECs) were investigated. After 0, 4, 8, 12 or 24 weeks of cryopreservation in liquid nitrogen, the HUVECs were thawed. The excretory funct...

Abstract Vascular inflammation activated by pro-inflammatory cytokines is an inflammatory response that occurs in the early stages of atherosclerosis. Endothelial dysfunction vascular begins with expression cell surface adhesion molecules cytokines. The purpose this study was to evaluate and verify effects isobavachalcone. In study, we investigated isobavachalcone on responses induced tumor nec...

Colorectal cancer (CRC) aggressiveness is caused by angiogenesis which promotes the growth and metastasis associated with poor prognosis survival. The vascular endothelial factor-A (VEGF-A) its receptor (VEGFR-2) form major signaling pathway in angiogenesis. This study aimed to investigate anti-angiogenesis activity of quercetin both colorectal cells cells. tube formation human vein (HUVECs) wa...

Docetaxel (DTX), a paclitaxel analogue, can efficiently inhibit proliferation of vascular smooth muscle cells and has broadly been used as an antiangiogenesis drug. However, as a candidate drug of drug-eluting stent, the effects of DTX on human umbilical vein endothelial cells (HUVECs) are still not well understood. Herein, we investigated the effects of DTX on proliferation, apoptosis, adhesio...

Histone deacetylases (HDACs) are key enzymes for post-translational modification and influence on various cellular activities. Thus, HDACs associated with many diseases their inhibitors have clinical significance. Here, 4-Hexylresorcinol (4HR) was studied as an inhibitor class I using the HDAC (HDACi) Trichostatin-A a positive control. The 4HR administered 1–100 μM to human umbilical endothelia...

Vaccination with xenogeneic and syngeneic endothelial cells is effective for inhibiting tumor growth. Nontoxic diphtheria toxin (CRM197), as an immunogen or as a specific inhibitor of heparin-binding EGF-like growth factor, has shown promising antitumor activity. Therefore, immunization with or administration of viable human umbilical vein endothelial cells (HUVECs) combined with CRM197 could ha...