Hyaluronidase is a goat testicular protein that hydrolyzes hyaluronic acid, a structural component of the intercellular matrix. It is commonly used as a spreading factor to improve the diffusion of drugs, including local anesthetics and chemotherapeutics. We experienced a 55-yr-old female with generalized urticaria that developed within 1 hr after the epidural injection of hyaluronidase. She ha...

Hyaluronan, a high molecular weight, negatively charged polysaccharide, is a major constituent of the extracellular matrix. High molecular weight hyaluronan is antiangiogenic, but its degradation by hyaluronidase generates proangiogenic breakdown products. Thus, by expression of hyaluronidase, cancer cells can tilt the angiogenic balance of their microenvironment. Indeed, hyaluronidase-mediated...

Hyaluronidase is generally used to remove cumulus cells from mouse oocytes before oocyte cryopreservation, intracytoplasmic sperm injection or DNA injection. In general, use of cumulus-free mouse oocytes decreases in vitro fertilizing ability compared with cumulus-surrounded oocytes. The effect of hyaluronidase exposure on the quality of mouse oocytes is not fully understood. Here, we investiga...

ABSTRACT Purpose The application of hyaluronidase in ophthalmology dates back 60 years, when it was first included in retrobulbar blocks. Now it is used routinely in retrobulbar, peribulbar, and sub-Tenon’s blocks. Until recently, the only sources of hyaluronidase were animal derived, either pharmaceutically manufactured or compounded. Animal-derived products have been associated with low purit...

INTRODUCTION Facial and neck hematomas are a significant complication of surgical procedures and direct trauma. Effective treatment of these hematomas is crucial for the prevention of cosmetic deformity, functional disturbance, infection, and tissue necrosis. Current treatment options include aspiration, incision and drainage, steroid injection, and drainage tube placement. However, these metho...

Loxoscelism is the designation given to clinical symptoms evoked by Loxosceles spider's bites. Clinical manifestations include skin necrosis with gravitational spreading and systemic disturbs. The venom contains several enzymatic toxins. Herein, we describe the cloning, expression, refolding and biological evaluation of a novel brown spider protein characterized as a hyaluronidase. Employing a ...

BACKGROUND Hyaluronidases are essential for the breakdown of hyaluronate (HA) in tissues and may be used to prevent the adverse effects of HA fillers. OBJECTIVES We explored the effect of hyaluronidase on exogenous and endogenous HA in vitro and in vivo. MATERIALS AND METHODS HA fillers were incubated with different concentrations of hyaluronidase and visualized by electrophoresis. HA fille...

Hyaluronidase is an enzyme that has temporary and reversible enzymatic effects on the matrix of connective tissue. When added to local anesthetics in pain treatments, it enhances their infiltration and dispersal into tissues. It is widely used in anesthesia for ocular, dental, and plastic surgery. Reports of drug hypersensitivity to hyaluronidase are rare and are usually confined to peribulbar ...

It has been reported that a polysaccharide extracted from Pseudomonas aeruginosa is depolymerized by bovine testicular hyaluronidase (Warren and Gray, 1954). In the course of studies dealing with the effect of hyaluronidase on the cell structure of the organism, it was noted that buffered suspensions of P. aeruginosa released appreciable amounts of the polysaccharide in the supernatant fluid wh...

Hyaluronidase has been gaining interest because it reduces tissue edema and fibrosis. Although rare, hyaluronidase has been shown to cause allergic reactions. A few cases of allergic reactions following hyaluronidase administration have been reported. Most of the described patients presented allergic reactions after peribulbar anesthesia for eye surgery. In this report, we describe three patien...