The protective efficacy of DNA plasmids encoding avian infectious bronchitis virus (IBV) S1, N, or M protein was investigated in chickens. Chickens were inoculated monovalently (with plasmid pVAX1-16S1, pVAX1-16M, or pVAX1-16N alone) or multivalently (combination of the three different plasmids, pVAX1-16S1/M/N). A prime-boost immunization protocol against IBV was developed. Chickens were immuni...

infectious bronchitis virus (ibv) is an acute and contagious viral disease of poultry that affects different systems, including the respiratory tract in particular. ibv causes major economic losses in the poultry industry globally. due to antigenic variation of the causative agent, control of the disease is difficult. to control the disease, many vaccines that belong to different serotypes are ...

It is important to rapidly differentiate infectious bronchitis virus (IBV) from disease agents like highly pathogenic avian influenza virus and exotic Newcastle disease virus, which can be extremely similar in the early stages of their pathogenesis. In this study, we report the development and testing of a real-time RT-PCR assay using a Taqman-labeled probe for early and rapid detection of IBV....

Colibacillosis results from infection with avian pathogenic Escherichia coli bacteria. Healthy broilers are resistant to inhaled E. coli, but previous infection with vaccine or virulent strains of Infectious Bronchitis Virus (IBV) predisposes birds for severe colibacillosis. We investigated whether IBV affects recruitment and function of phagocytic cells and examined NO production, phagocytic a...

280 Characterization of infectious bronchitis virus isolates from backyard flocks. AAAP abstract † 281 Recombination in avian gamma-coronavirus infectious bronchitis virus (IBV). 282 Evaluation of possible interference between Arkansas and Massachusetts vaccine serotypes. AAAP abstract † 283 Interactions between multivalent attenuated live infectious bronchitis virus (IBV) vaccines in one-day-o...

OBJECTIVE To monitor and study the molecular epidemiology, evolution and pathogenicity of infectious bronchitis viruses (IBVs) in China in recent years and further our knowledge of the evolution of IBVs. METHODS Thirty-seven IBV isolates were isolated from commercial chickens in China. The isolates were characterized by RT-PCR, sequencing, typing and analyzing the entire S1 gene. In addition,...

The avian coronavirus infectious bronchitis virus (IBV) is the causative agent of the respiratory disease infectious bronchitis of domestic fowl, and is controlled by routine vaccination. To explore the potential use of IBV as a vaccine vector a reverse genetics system was utilised to generate infectious recombinant IBVs (rIBVs) expressing the reporter genes enhanced green fluorescent protein (...

Infectious bronchitis virus (IBV) can multiply effectively in chick embryo kidney (CEK) cells after adapting to the chick embryo. To investigate the dynamic changes in IBV load in the supernatant of primary CEK cells, we developed an SYBR Green I-based real-time polymerase chain reaction assay to quantify nucleic copy numbers of the IBV-Sczy3 strain. The 20, 54, and 87th generations of CEK-adap...

Infectious bronchitis virus (IBV) has a variety of serotypes with relatively limited cross-protection leading the disease to be a major problem in the poultry industry. The IBV 793/B strain has identified to circulate in Iran; therefore, the development of a specific vaccine to protect against the virulent virus has received attention. In this regard, the live IB 793/B vaccine (793/B.08IR) was ...

Background and Aim: QX-like infectious bronchitis virus (IBV) is a highly avian coronavirus that causes respiratory kidney disease. It linked to increased mortality loss of performance in infected chickens worldwide, including Thailand. Thus, simple rapid diagnostic method for the diagnosis IBV needed. This study aimed develop single-step multiplex reverse transcription-polymerase chain reactio...