Counting cells is a crucial procedure in evaluating the success of a treatment. A fast yet exact way of doing this for DAPI-stained cells with the ImageJ-derivate ‘Fiji’ is described in this paper, written as a step-by-step tutorial with screenshots.

Up to now online image processing in the browser could be done in two ways: It could be achieved using Java applets or a Flash component. However both approaches needed a browser plugin, which is not available for all platforms. Now with the new HTML5 canvas element, which will soon be supported by most browsers, image processing tasks can be achieved using JavaScript only. This paper will expl...

Abstract This chapter introduces GPU-accelerated image processing in ImageJ/Fiji. The reader is expected to have some pre-existing knowledge of ImageJ Macro programming. Core concepts such as variables, for -loops, and functions are essential. provides basic guidelines improved performance typical workflows.

In order to reduce herbicide application an intelligent sprayer boom is being developed. It only sprays with herbicides if the weed infestation exceeds a certain weed control threshold. The estimation of leaf cover of weeds through image analysis is a prerequisite for the weed management model of the intelligent sprayer boom. Destructive and human perception methods of leaf cover estimation are...

In this paper, we introduce the centerline extraction processes of the liver vessel systems by using the plug-ins in ImageJ and 3D Slicer. The Skeletonize (2D/3D) plug-in in ImageJ is easy to use, and only based on the volumetric data. It performs fast and require no user interactions in terms of seed points selection. The VMTK toolkit in 3D slicer is more powerful. It uses the surface model an...

Intentional and unintentional Oil spill in the ocean is significantly harmful to marine life, coastal area and whole environment. There are many softwares available which can detect oil spill but they are not application specific and some of them are paid. We have implemented feature extraction for oil spill detection using imageJ tool. This paper gives implementation details and results of fea...

Magnetic resonance elastography (MRE) is a promising method for health evaluation and disease diagnosis. It makes use of elastic waves as a virtual probe to quantify soft tissue elasticity. The wave actuator, imaging modality and elasticity interpreter are all essential components for an MRE system. Efforts have been made to develop more effective actuating mechanisms, imaging protocols and rec...

BACKGROUND AND AIMS Although many methods exist for quantifying the number of pollen grains in a sample, there are few standard methods that are user-friendly, inexpensive and reliable. The present contribution describes a new method of counting pollen using readily available, free image processing and analysis software. METHODS Pollen was collected from anthers of two species, Carduus acanth...