The presence of minute amounts of embryonic -globin chains in adult hemolysates is a marker for carriers of a-thalassemia-1 resulting from ( / ) deletion. Recently. we developed a murine monoclonal antihuman embryonic -globin chain antibody, 8E8. By using this antibody. we have now established a slot-blot immunobinding assay for the rapid detection of -globin chains in adult hemolysates. -globi...

A procedure is described for the routine laboratory diagnosis of viral serum antibodies. Antigens are dotted on nitrocellulose strips or sheets, and sera are applied on absorbent paper strips. Antigen-antibody complexes are detected with enzyme-conjugated antiglobulin and development of a colored, insoluble substrate product. The test allows processing of multiple sera in one 3- to 5-h operatio...

In this study, a dot-immunobinding assay (Dot-Iba) was standardized to measure circulating antimycobacterial antibodies in the cerebrospinal fluid (CSF) specimens for the rapid laboratory diagnosis of tuberculous meningitis (TBM). Specific CSF-IgG antibody to Mycobacterium tuberculosis from a culture proven patient with TBM was isolated and coupled with activated Cynogen bromide-Sepharose 4B. U...