Purpose: To investigate the mechanism of action and effects abietic acid (AA) in a mouse acute lung injury (ALI) model.
 Methods: A ALI model was established by lipopolysaccharide (LPS) induction. Lung tissues were examined for histological alterations scored based on degree injury. Myeloperoxidase (MPO), IL-6, IL-1β, TNF-α levels measured enzyme-linked immunosorbent assay (ELISA) while nu...

Co-immunoprecipitation of antigens from cell or tissue extracts is used in a wide variety of experimental systems to corroborate the existence of authentic protein-protein interactions in vivo. Typically, stable protein-protein associations are detected by immunoprecipitation with antibodies specific for one protein, followed by immunoblot analysis with antibodies against potential interacting ...