Immunocytochemistry is a very powerful and fairly straightforward method for determining the presence, subcellular localization, and relative abundance of an antigen of interest, most commonly a protein, in cultured cells. This protocol presents an easy-to-follow series of steps that will enable researchers to conserve primary and secondary antibodies while getting high quality, reproducible qu...

0

Immunostaining can be used to pinpont the subcellular localization of a protein antigen, to follow its changing position as cells respond to stumuli, or to compare its position to other proteins in the same cell. Using these methods one can follow an antigen’s distribution during development, mark the location of a particular cell type in a multicellular in vivo setting, or determine the presen...

Immunocytochemistry, the identification of cell- or tissue-bound antigens in situ, by means of a specific antibody-antigen reaction, tagged microscopically by a visible label, has a remarkably wide range of applications. The basic techniques are straightforward and can be adapted to explore the localisation of virtually any molecule of interest to the researcher in samples of normal and/or mali...