Eleven commercial kits for serum ferritin have been compared. Seven kits used RIA and four IRMA methodology. Seven kits used human liver ferritin, two human spleen ferritin and two undefined human ferritin as standard material. Incubation times varied between 30 minutes and 36 hours. Only four kits gave normal ranges determined with the kit in question. All values were compared with the kit in ...

A cross-sectional study of thyrotropin (TSH), thyroxine (T4) and triiodothyronine (T3) in human serum was performed aiming to define their normal values. In total, 201 assays were performed on 70 normal individuals, 26 men and 44 women, with ages ranging from 2 to 84 years. Serum TSH was measured with a highly sensitive immunoradiometric assay (IRMA) kit and T4 & T3 were determined with commerc...

CALCITONIN gene-related peptide (CGRP), a 37amino-acid neuropeptide identified as a potent vasodilator [1], is widely distributed in the central nervous system. The presence of CGRP and its binding sites has been found in the pituitary [2-4], but its role in regulating pituitary function remains to be determined. In the present study, we investigated the effect of CGRP on the secretion of GH in...

Prostate cancer is the sixth most common cancer in the world, the third most common cancer in men. In 2000, the number of new cases of prostate cancer was estimated at 513000 worldwide and this disease accounts for 9.7% of all cancers in men (1, 2). Thus early detection and local treatment have been advocated in an effort to influence the significant morbidity and mortality associated with the ...

BACKGROUND & OBJECTIVES Estimation of parathyroid hormone (PTH) levels is important in the management of metabolic bone disorders. Here we describe a simple, sensitive and specific second generation immunoradiometric assay (IRMA) to detect intact PTH levels using different solid phase matrices. Different methods for immobilization of antibodies have also been evaluated. METHODS Experiments we...

Elevation of serum concentrations of soluble interleukin-2 receptor (sIL-2R) has been shown in various hematolymphoid malignancies.'" Since its first documentation by Komp et alx in 1989, sIL2R has also been measured and tested for clinical usefulness as a prognostic indicator for patients with hemophagocytic lymphohistiocytosis (HLH)?"' However, these studies evaluated a small number of patien...

Elevation of serum concentrations of soluble interleukin-2 receptor (sIL-2R) has been shown in various hematolymphoid malignancies.'" Since its first documentation by Komp et alx in 1989, sIL2R has also been measured and tested for clinical usefulness as a prognostic indicator for patients with hemophagocytic lymphohistiocytosis (HLH)?"' However, these studies evaluated a small number of patien...

To evaluate the usefulness of CYFRA 21-1 and SCC Ag in the diagnosis of squamous cell carcinoma (SQC) of the lung, we tested sera from 124 patients with lung cancers (squamous cell ca 72, adenoca 22, large cell ca 4, small cell ca 18 and undetermined 8) and 78 patients with inflammatory lung diseases (bronchitis 24, bronchiectasis 29, tuberculosis 19 and others 6) using immunoradiometric assay ...

We compared the utility of a sensitive immunoradiometric assay for serum thyrotropin as a "first-line" thyroid-function test with a strategy based on first measuring total thyroxin in serum. The immunoradiometric assay appears to distinguish primary hypothyroidism and hyperthyroidism from euthyroidism in "new" patients. The role of this test in monitoring antithyroid treatment or thyroxin-repla...

We herein report familial defects in the molecular structure of luteinizing hormone (LH). The propositus was a 29-year-old women with repeated abortion in whom the serum LH concentration was extremely low determined by an immunoradiometric assay utilizing two monoclonal antibodies for the intact LH dimer and beta-subunit of LH (SPAC-S kit). Further studies on the serum LH concentration in this ...