MOTIVATION We developed a technique and a tool for degenerate primer design based on multiple local alignments employing the MEME algorithm supported with electronic PCR. The objective is to find adequate primers starting from sequences with poor global similarity. We show an example of its application in our laboratory to find sequences in Brachiaria with similarity to ESTs related to apomixis.

2. Objectives ........................................................................................................... 4 2.a. Engineering a new Industry...................................................................................... 4 2.b. Mark the territory: the stakeholders .......................................................................... 5 2.c. Identify the 'issues' ..........

Multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of RNA interference (RNAi) cassettes. Although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. Here, we worked out a simple cloning stra...

it has been suggested that single nucleotide polymorphisms (snps) in genes involved in toll-like receptors (tlrs) pathway may exhibit broad effects on function of this network and might contribute to a range of human diseases. however, the extent to which these variations affect tlr signaling is not well understood. in this study, we adopted a bioinformatics approach to predict the consequences...

We present here a technique for correlating T-RFLP fingerprints with genomic clone libraries, using a in silico restriction analysis, in order to identify the microbial (Bacteria and Archaea) communities present in an environmental sample. This technique is very useful for independent of culture metagenomic studies. We also show some results on the application of this technique to 3 environment...

The exponential expansion of the publicly available human DNA sequence database has increasingly facilitated cloning by homology of genes for biochemically defined, functionally similar proteins. We hypothesized that an as-yet uncloned human alpha-glucosidase (human neutral alpha-glucosidase C or GANC) is a previously uncharacterized member of a paralogous human glycosyl hydrolase gene family 3...

human cytomegalovirus is one of the most common pathogenic viruses all over the world. in congenital infection leads to neurologic severe disorders and even death of fetus and in individuals with immunosuppression may also cause severe clinical symptoms. multiple evidence indicate that among several strategies, epitope-based vaccine (evs) that can induce both humoral and cellular immunity respo...

the properties relevant to pharmacokinetics and pharmacodynamics of four series of synthesized s-triazine derivatives have been studied by quantitative structure-retention relationship (qsrr) approach. the chromatographic behavior of these compounds was investigated by using reversed-phase high performance thin-layer chromatography (rp-hptlc). chromatographic retention (rm0) was correlated with...