objective: electroporation is the most common method used for the transfection of dna. although this method has been attributed for various cell using different buffer compositions, the effects of dna concentration on the transfection efficiency has not yet been studied. here the correlation between the efficiency of electroporation reaction and increments of dna concentration has been investig...

background: parental anxiety while waiting for the results of amniocentesis has been investigated by many authors. it seems that the implementation of faster techniques such as fluorescence in-situ hybridization (fish) will have some benefits in reducing this anxiety. besides the patients' attitudes to choosing this method, gynecologists who are the persons responsible for treatment, must feel ...

Background Selecting the best embryo for transfer, with the highest chance of achieving a vital pregnancy, is a major goal in current in vitro fertilization (IVF) technology. The high rate of embryonic developmental arrest during IVF treatment is one of the limitations in achieving this goal. Chromosomal abnormalities are possibly linked with chromosomal arrest and selection against abnormal fe...

BACKGROUND Malignant lymphomas are classified based on morphology, immunophenotype, genetics and clinical features. The pathological diagnosis is generally considered difficult and prone to mistakes. Since non-random chromosomal translocations are specifically involved in specific entities, their detection is an important adjunct for increasing the reliability of the diagnosis. Recently, split-...

breast cancer is preservative of the genetic heterogeneous alterations. in the present investigation, fluorescence in situ hybridization (fish) was performed on the fresh primary tumours of 6 patients with infilterating ductal carcinoma. dna-probe for centromere of chromosome 3 (3cen) was applied and in the total analysis, the majority of interphases presented 1 signal (33.6%) which followed by...

AIMS Fluorescence in situ hybridization (FISH) can be used to reveal several genomic imbalances relevant to proper cancer diagnosis and to the correct treatment regime. However, FISH requires expensive and advanced fluorescence microscopes in addition to expertise in fluorescence microscopy. To determine whether a newly developed dual-colour chromogenic in situ hybridization (CISH) method is a ...