An efficient micropropagation protocol of Bacopa monnieri (L.) Pennell through two-stage culture nodal segments and ex vitro acclimatizationShashikanta Behera, Nibedita Nayak, Shasmita, Durga P. Barik, Soumendra K. Naik

The capacity to control developmental and morphogenetic processes in vitro has always been the fascination and primary focus of many ornamental tissue culture studies. Thin cell layers or TCLs were first used to control the development of flowers, roots, shoots and somatic embryos in tobacco pedicels. Since those studies over 30 years ago, TCLs have been successfully used in the micropropagatio...

In micropropagation of lily, preferably bulblets should be produced: Because bulblets are compact and robust, they are much easier to handle and to plant in soil than shoots. In this review, the various factors that determine bulblet growth in vitro are discussed. Gibberellins, jasmonates (JA) and abscisic acid (ABA) are the major identified plant growth regulators (PGRs) for storage organ form...

Galactomannans (GMs) extracted from seeds of a native Brazilian specie designated Cassia fastuosa (cassia) and from Cyamopsis tetragonolobus (guar gum – a commercial GM) were mixed with agar, in the proportion of 3/3 g.L (w/w), and used as a gelling agent in Marubakaido apple rootstock (Malus prunifolia Borkh) micropropagation. The rheological measurements of the gels showed a good interaction ...

In vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. Micropropagation using nodal segments of Rosa canina under different combinations of BAP, GA3 and NAA on MS and VS, medium was investigated. The results showed that the highest shoot proliferation was obtained on VS medium cont...

Micropropagation is an important technique for rapid and large-scale multiplication of selected plants. The type and concentration of plant growth regulators and physiological status of the explant have profound effect on the efficacy of plant regeneration. However, success of any micropropagation protocol depends upon the percentage survival and successful establishment of plants from in vitro...

Different in vitro culture techniques have been used for rapid plant propagation. Pathogenfree plants have been produced using techniques such as seed culture, meristem culture, micropropagation using axillary or adventitious shoot buds, and somatic embryogenesis. This Bulletin discusses the importance of in vitro culture techniques for the production of pathogen-free medicinal plants such as A...