An in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by KC medium. After 40 days, all the media turned brown and the growths of the protocorms were arrested. Activated charcoal, 1 g/l ...

Conventional in vitro propagation may affect normal morphophysiological characteristics and survival during the acclimatization of commercial plants, such as Etlingera Elatior (Jack) R.M. Smith. CO2 enrichment, gas-permeable membranes, reduced carbohydrate concentration culture media can be used to mitigate these effects. Here, we aimed determine impact photomixotrophic photoautotrophic growth ...

Plectranthus amboinicus (Lour.) Spreng is a perennial plant from the mint family with aromatic, succulent leaves and several health benefits. Multiple shoot regeneration was accomplished in vitro using nodal segments (NS) explants of P. pretreated 0, 0.5, 5, 25, 50, 100 μM thidiazuron (TDZ) for 4 h, then transferred to growth regulator-free media. After 8 weeks growth, NS pre-treated 25 TDZ h T...