The association between Escherichia coli endotoxin-induced organ damage and nitric oxide-related mechanisms was investigated in the spleen of male Swiss albino mice (20-40 g) by using (1) Pt/Ir electrochemical sensor connected to an amperometric detection system (NO-501, InterMedical Co., Japan), (2) nitrotyrosine immunohistochemistry, (3) conventional light microscopy and (4) immunoblotting te...

background: the aim of this study was to compare the effects of diode laser irradiation on wound healing in oral rat mucosa and also to measure the amount of inducible nitric oxide synthase (inos) and endothelial nos (enos) on oral wound healing. healing was assessed by histology and the amounts of enos and inos were measured by real‑time polymerase chain reaction (pcr). materials and methods: ...

The objective of this study was to investigate the effects of selective inducible nitric oxide synthase and neuronal nitric oxide synthase inhibitors on cartilage regeneration. The study involved 27 Wistar rats that were divided into five groups. On Day 1, both knees of 3 rats were resected and placed in a formalin solution as a control group. The remaining 24 rats were separated into 4 groups,...

BH4: Tetrahydrobiopterin; BH2: Dihydrobiopterin; NOS: Nitric Oxide Synthase; eNOS: endothelial Noxide Synthase; nNOS: Neuronal Nitric Oxide Synthase; iNOS: Inducible Nitric Oxide Synthase; CAD: Coronary Artery Disease; LC-MS/MS: Liquid Chromatography and Tandem Mass Spectrometry; GTCH1: Guanine Triphosphate Cyclohydrolase; NOS3: Nitrous Oxide Synthase 3 – Endothelial; QDPR: Quinoid Dihydropteri...

Previous studies have suggested that salt-sensitive hypertension in humans and experimental animals may in part be due to dysregulation of the L-arginine/nitric oxide system. This study was conducted to determine the endothelial, inducible, and neuronal nitric oxide synthase expressions in the kidney, heart, aorta, and brain of salt-sensitive and salt-resistant Dahl rats. We studied salt-sensit...

Objective(s):Some pathologic situations such as diabetes and metabolic syndrome are associated with alternation in nitric oxide level. Incidence of these condition increases with aging. On the other hand, insulin secretion is modulated by nitric oxide, and nitric oxide synthase (NOS) activity is also altered in diabetes. In this study, modification in the enzyme activity associated with aging a...

Excess production of nitric oxide by activated macrophages via inducible nitric oxide synthase leads to the development of various inflammatory diseases. Heme oxygenase-1 expression via activation of nuclear factor-erythroid 2-related factor 2 inhibits nitric oxide production and inducible nitric oxide synthase expression in activated macrophages. Okanin is one of the most abundant chalcones fo...

endo-derived nitric oxide (no) is synthesized from l-arginine by endothelium nitric oxide synthase (enos). since reduced no synthesis has been implicated in the development of coronary atherosclerosis; we hypothesized that polymorphisms of nos gene might be associated with increased susceptibility to this disorder and coronary artery disease (cad). we studied the 27 base pair tandem repeat poly...

A healthy endothelium is essential for vascular homeostasis, and preservation of endothelial cell function is critical for maintaining transplant allograft function. Damage to the microvascular endothelial cells is now regarded as a characteristic feature of acute vascular rejection, an important predictor of graft loss. It is also linked with transplant vasculopathy, often associated with chro...

Cyclosporine toxicity mainly affects kidney and liver function. We have previously shown that cyclosporine nephrotoxicity alters kidney nitric oxide synthase mRNA pattern of expression. To determine if nitric oxide synthase expression changes are mediated directly by cyclosporine or by secondary hemodynamic alterations induced by cyclosporine, we evaluated if these effects are tissue specific a...