To detect the presence of infectious bronchitis virus (IBV) in infected allantoic fluid (AF) of SPF embryonated eggs rapid hemagglutination (HA) activity after treatment with neuraminidase enzyme was used. Twenty IBV suspected materials were inoculated in SPF embryonated eggs via chorioallantoic cavity. Harvested AFs were treated with neuraminidase enzyme and the presence of IBV was detected by...

rapid detection and differentiation of infectious bronchitis virus (ibv) involved in the disease outbreak is very important for controlling disease and developing new vaccines. in the present study, three regions of the genome of ibv vaccine and field isolates including s1 gene, gene 3 and nucleocapsid (n) gene along with 3' untranslated region (3' utr) were amplified and subjected to restricti...

Infectious bronchitis (IB) is a highly contagious disease of the respiratory and urogenital tract of chickens, caused by infectious bronchitis virus (IBV), a member of the family Coronaviridae. The disease is common throughout the world where chickens are produced commercially. PCR on reverse transcribed RNA is a potent technique for the detection of IBV. In comparison with classical detection ...

BACKGROUND: Avian infectious bronchitis is considered as an important viral disease worldwide. Genotyping based on the S1 subunit of spike protein gene of the causative agent, avian infectious bronchitis virus, can be used to classify IBV isolates. Objective: This survey was carried out to characterize the infectious bronchitis virus (IBV) genotypes circulating in Iran and determine their preva...

to detect the presence of infectious bronchitis virus (ibv) in infected allantoic fluid (af) of spf embryonated eggs rapid hemagglutination (ha) activity after treatment with neuraminidase enzyme was used. twenty ibv suspected materials were inoculated in spf embryonated eggs via chorioallantoic cavity. harvested afs were treated with neuraminidase enzyme and the presence of ibv was detected by...

A study has been carried out for determining of cross-immunity between Massachusetts (Mass) M41 and Iranian IR/773/2001 (793/B) strains of infectious bronchitis virus. Two groups of 20 three weeks old SPF chickens vaccinated subcutaneously with two oil-emulsions inactivated Infectious Bronchitis Vaccines (Mass M41 and Iranian IR/773/2001 strains) that had been manufactured in Razi institute in ...

Infectious bronchitis virus (IBV), the coronavirus of the chicken (Gallus gallus), is one of the foremost causes of economic loss within the poultry industry, affecting the performance of both meat-type and egg-laying birds. The virus replicates not only in the epithelium of upper and lower respiratory tract tissues, but also in many tissues along the alimentary tract and elsewhere e.g. kidney,...

The detection methods for infectious bronchitis virus (IBV) are reviewed. Advantages and disadvantages of available techniques of IBV detection by virus isolation, antigen or genome detection, and serology are discussed. Factors of influence on the level of success in detection of IBV after a disease outbreak are discussed, as are the possibilities and dangers of strain classification by protec...