objective(s) lipocalin 2 (lcn2) is a 25-kda glycoprotein that has initially been extracted from neutrophil granules. expression of lcn2 is induced under various pathophysiological conditions. it is also known as an early marker of kidney and heart injury. high-level expression of recombinant lcn2 neutrophil gelatinase-associated(ngal) in insect cells was the aim of this study. materials and met...

Effects of conditioned media prepared from cell lines derived from 11 insect species (six families, three orders) on the in vitro growth and development of the egg parasitoid Edovum puttleri were investigated. The parasitoid exhibited significantly different responses to the various insect cell line-conditioned media that were incorporated into the artificial diets. When cell lines were derived...

The number of nucleoli in a cell and nucleolar area vary according to the cell. We compared nucleoli in mammalian circulating lymphocytes and insect circulating haemocytes. An increased nucleolar coefficient correlated with a lowered nucleoli size. The smaller nucleolar size in mammalian lymphocytes indicates a lower proteosynthetic cellular activity in both mammalian lymphocytes and insect hae...

Cochineal is the name of both crimson or carmine dye and the cochineal insect (Dactylopius coccus), a scale insect from which the dye is derived. There are other species in the genus Dactylopius which can be used to produce cochineal extract, but they are extremely difficult to distinguish from D. coccus, even for expert taxonomists, and the latter scientific name (and the use of the term "coch...

The culture of insect tissues in vitro offers considerable potential for studying many aspects of the pathogenic cycles of protozoan, bacterial, and viral agents which have an insect vector or reservoir . Studies directed toward this end have been limited in number since the culture of insect cells has, in itself, been a very difficult task (2, 8, 13) . Progress in this field was not too encour...

Ethiopia is remarkably rich in its biological, ecological and landscape diversity and is home to outstanding natural bio-resources such as a number of herbs, aromatic and medicinal plants. The present study was carried out to study the composition, distribution and economic importance of insect pests of prioritized aromatic (Mentha sp., lemon verbena, fennel, rosemary, rosadamascene, oregano, p...

reteplase is a potent thrombolytic agent which is widely used in the management of acute myocardial infarction and stroke. it belongs to the third generation of the thrombolytic drugs and has been derived from native human tissue plasminogen activator by removing three domains of it and keeping the kringle 2 and serine protease domains. however, the high cost of this drug, has limited the appli...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...