An in vitro culture system of primary cells from white-backed planthopper, an insect vector of Southern rice black-streaked dwarf virus (SRBSDV), a fijivirus, was established to study replication of the virus. Viroplasms, putative sites of viral replication, contained the nonstructural viral protein P9-1, viral RNA, outer-capsid proteins, and viral particles in virus-infected cultured insect ve...

Background and Aims: Newcastle disease virus (NDV) is one of the major pathogen in poultry. Vaccination is intended to control the disease as an effective solution nevertheless this virus is a growing threat to the poultry industry. F gene open reading frame (ORF) from NDV is 1650 bp, encoding a protein of 553 amino acids that can induce protective immunity alone. The F glycoprotein on the surf...

BACKGROUND: West Nile virus (WNV) is a vector-borneagent that is maintained within a bird-mosquito cycle. In humansand equids, infection by this agent is usually asymptomatic, orcharacterized by a mild febrile illness. However, fatal meningoencephalitisor encephalitis may occur. OBJECTIVES:The aim ofthis study was to evaluate the prevalence of WNVinfection andcorrelation of this organism with h...

To prevent epidemics of mosquito-transmitted diseases like Chikungunya in Réunion Island, we develop tools to control its principal vector, Aedes albopictus. Biological control tools, like the Sterile Insect Technique (SIT), are of great interest as an alternative to chemical control tools which are very detrimental to environment. The success of SIT is based on a good knowledge of the biology ...

Rice dwarf virus (RDV) replicates in and is transmitted by a leafhopper vector in a persistent-propagative manner. Previous cytopathologic and genetic data revealed that tubular structures, constructed by the nonstructural viral protein Pns10, contain viral particles and are directly involved in the intercellular spread of RDV among cultured leafhopper cells. Here, we demonstrated that RDV expl...

Control of insect vector populations is an integral part of disease management but has many challenges. Area-wide campaigns, mainly based on insecticide administration, are most effective for control of insect populations, whereas disease prevention is more localised and protects a smaller number of animals against insect vector contact. Various control and prevention techniques are available f...

The principal families of insect vectors which cause the most damage to agricultural crops through the spread of plant diseases are in the order Hemiptera, and include the aphids, leafhoppers, delphacid planthoppers and whiteflies. Another important group of insect vectors found worldwide is the order Thysanoptera, the thrips. Other insects also spread plant diseases; however, aphids alone are ...

The culture of insect tissues in vitro offers considerable potential for studying many aspects of the pathogenic cycles of protozoan, bacterial, and viral agents which have an insect vector or reservoir . Studies directed toward this end have been limited in number since the culture of insect cells has, in itself, been a very difficult task (2, 8, 13) . Progress in this field was not too encour...

BACKGROUND Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. METHODS The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...