Purpose: This study sought (i) to investigate the relationship between Postwash total motile sperm count and Postwash percentage motile sperm in predicting successful intrauterine insemination and (ii) to determine the minimal Postwash total motile sperm count required to achieve pregnancy with intrauterine insemination. Methods: Five hundred four women, who underwent 1636 intrauterine insemina...

In an experiment involving the artificial insemination of 312 Merino ewes, fertilization, early (18 day), and late (100 day) pregnancy rates were examined after intrauterine insemination with frozen semen using a laparoscope. Oestrus was synchronized in ewes by progestagen sponge/PMSG treatment. After cervical insemination of fresh semen (control) 55 hrs after sponge removal, the fertilization,...

Blood serum progesterone (P4) levels in donor cows and heifers at donor selection, superovulation, insemination and embryo recovery were investigated. Significant differences in P4 levels in the investigated donors during superovulatory oestrus insemination were observed. The cows with low levels of P4 at insemination (5.8 ± 1.7 nmol/l) showed a better yield and quality of embryos (P<0.01) comp...

Pheromones mediate social interactions among individuals in a wide variety of species, from yeast to mammals. In social insects such as honey bees, pheromone communication systems can be extraordinarily complex and serve to coordinate behaviors among many individuals. One of the primary mediators of social behavior and organization in honey bee colonies is queen pheromone, which is produced by ...

In this case, semen that handled by urethral catheterization was deposited into the deepest point of vagina an anesthetized queen. Intravaginal artificial insemination applied on 2nd day onset oestrus and only single performed. Insemination time determined behavioural cytological evaluations. Two healthy kittens were obtained 62 days after insemination. The case has important reports in terms b...

The aim of this research was to estimate the influence of environmental factors, such as air temperature and milk production, on reproduction traits of primiparous Polish Holstein-Friesian cows. The following reproduction traits were considered: interval from calving to first insemination, interval from first to last insemination, interval from calving to conception, number of inseminations per...

In normal production practices, sows and gilts are inseminated at least twice during estrus because the timing of ovulation is variable relative to the onset of estrus. The objective of this study was to determine if a normal fertilization rate could be achieved with a single insemination of low sperm number given at a precise interval relative to ovulation. Gilts (n=59) were randomly assigned ...

The objective of the present field study was to establish the beneficial effects of re-insemination of non-pregnant cows using ultrasonography 20 to 23 days after the artificial insemination. A total of 245 Japanese Black cows were artificially inseminated and early pregnancy diagnosis (EPD) was performed on 92 cows 20 days after insemination, using ultrasonography. The remaining 153 cows were ...

The present study was performed to assess the fertility of frozen-thawed dog semen prepared by freezing with 6% glycerol and thawing at 70 degrees C for 8 sec, and to evaluate the least number of post-thaw spermatozoa necessary to achieve pregnancy by intrauterine or intratubal artificial insemination. It was found that the pregnancy rate of intrauterine artificial insemination was 100% using 6...

The objective was to determine whether granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates development of bovine embryos. In each experiment, oocytes were fertilized in vitro, GM-CSF was added to embryo culture medium at 8-10 h or 5 days after insemination, and development was monitored as the proportion of oocytes that formed blastocysts. Addition of recombinant bovine GM-CSF ...