objective(s) in addition to several molecular methods and in particular 16s rdna analysis, the application of a more discriminatory genetic marker, i.e., 16s-23s internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. in the current study we aimed to apply this sequencing power to conclusive identification of some iranian clinical s...

To study the phylogenetic relationships between Erysiphe s. lat. and Microsphaera, the nucleotide sequences of internal transcribed spacers ofrDNA including 5.8S rDNA gene were determined for 23 taxa. The results showed that Erysiphe. section Erysiphe and Microsphaera are closely related and clustered together with strong bootstrap support (100%). All oftaxa belonging to this group produce coni...

Various molecular techniques like analysis of the amplified rDNA internal transcribed spacers (ITS), intragenic spacers and total ITS region analysis by restriction fragment length polymorphism (RFLP) has been introduced for yeast identification but there are limited databases to identify yeast species on the basis of 5.8S rDNA. In this study, twenty nine yeast strains from various sources incl...

Polymerase chain reaction-linked restriction fragment length polymorphism (PCR-RFLP) analysis of ribosomal (r) DNA was conducted on Uncinaria stenocephala, Ancylostoma caninum, A. tubaeforme and A. ceylanicum. The rDNA region spanning the first and second internal transcribed spacers (ITS1 and ITS2) plus the 5.8S (ITS+) gene was amplified by PCR from each of the species, digested separately wit...

Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical ...

The ribosomal DNA (rDNA) cluster of insects contains several hundred repeating structural-functional units and, therefore, is a typical example of a multigene family. Eukaryotic ribosomal RNA (rRNA) genes (18S, 5.8S, and 28S like) are arranged in tandemly repeated clusters in the nucleolus organizers, separated by several spacers, namely the nontranscribed spacer, the external transcribed space...

the genus phaeoacremonium is associated with decline disease of woody plants and with human infections. members of this genus have broad host range and wide geographical distribution. during 2010, ten isolates of phaeoacremonium were recovered from vascular tissues of persimmon (diospyros kaki) trees, showing decline symptoms in shiraz city. anamorphic characteristics such as, conidiophore morp...

The rDNA-ITS (Ribosomal DNA Internal Transcribed Spacers) fragment of the genomic DNA of 8 wild edible mushrooms (collected from Eastern Chota Nagpur Plateau of West Bengal, India) was amplified using ITS1 (Internal Transcribed Spacers 1) and ITS2 primers and subjected to nucleotide sequence determination for identification of mushrooms as mentioned. The sequences were aligned using ClustalW so...

Background and Purpose: By using advanced detection/identification methods, the list of emerging uncommon opportunistic yeast infections is rapidly expanding worldwide. Our aim in the present study was sequence-based species delineation of previously unidentified yeasts obtained from a clinically yeast collection. Materials and Methods: A total of twenty three out of the 855 (5.7%) yeast isolat...

dunaliella is a green halotolerant microalga, which has several industrial applications e.g. β-carotene production. identification of different dunaliella species has been carried out by morpho-physiological and recently molecular studies. to achieve an improved understanding of taxonomy, these studies are required to be in linkage. the present study describes molecular and specific morpho-phys...